Orientadores: Silvana Pereira Barros, Jose Merzel / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba / Made available in DSpace on 2018-08-05T13:43:16Z (GMT). No. of bitstreams: 1 Silva_MarcoAntonioDiasda_D.pdf: 2250245 bytes, checksum: 22329a07ea6108d7bacfd889fc338a72 (MD5) Previous issue date: 2005 / Resumo: O propósito deste estudo foi avaliar a influência do tratamento com doses intermitentes PTH na morfologia e na quantidade das fibras de Sharpey de dentes de crescimento contínuo em diferentes condições funcionais e na ultraestrutura dos hepatócitos de ratos. Os animais foram preparados de forma a apresentar normo, hipo e hiperfunção. O grupo tratado recebeu 40¿um¿g/kg PTH três vezes por semana, durante trinta dias e o grupo controle recebeu a mesma dose de veículo (ac. acético 1%). A) Utilizando microscopia eletrônica de varredura, foi avaliada a densidade de fibras de Sharpey na face lingual do alvéolo de incisivos. Os resultados demonstraram que os animais tratados com PTH, nas três condições funcionais apresentam uma maior densidade das fibras de Sharpey em relação aos respectivos controles.A mesma diferença se observa quando se considera apenas as fibras de Sharpey mineralizadas. B) No fígado cortes semifinos e ultrafinos foram analisados, através de microscopia de luz e eletrônica de transmissão e obtida a densidade volumétrica de hepatócitos e organelas. Foi demonstrado que os hepatócitos dos animais tratados com PTH apresentam aumento do retículo liso e diminuição da reserva deglicogênio. Os resultados indicam que doses intermitentes de PTH administradas por um período de 30 dias promovem um aumento da densidade de área das fibras de Sharpey em particular das totalmente mineralizadas, o que pode ter resultado no aumento da resistência do ligamento periodontal o que explicaria o retardo da velocidade eruptiva observado em animais tratados. As alterações ultraestruturais descritas nos hepatócitos indicam um aumento no metabolismo hepático promovido pelo hormônio / Abstract: The aim of this study was to evaluate the effects of PTH intermittent treatment on periodontal Sharpey fibers and hepatocytes ultrastructural components of wistar rats. The animals were divided in groups and exposed to different occlusaI forces, normal occlusion, hyperfunction and hypofunction. PTH treated animais received 40 'mu¿g/Kg of PTH, three times a week, during thirty days and the rats from control group received vehicle in following the same protocol. Sharpey fibers density was evaIuated through scanning electron microscopy in the lingual face of lower incisors. We observed that PlH treated animaIs presented increased density of Sharpey fibers and more mineralized fibers, in the three occ1usal conditions analyzed, when compared to the control. Transmission electronic microscopy morphometric anaIysis demonstrated that hepatocytes from PTH treated animals presented an increase of smooth endoplasmatic reticulum and a decrease on glycogen volumetric density. These results indicate that thirty days of PTH intermittent administration are responsible by an increase in aIveolar Sharpey fibers density and this evidence might be associated to an enhancement of periodontal ligament resistance and consequently to reduced eruptive rates previously observed. In hepatic tissue the increase in smooth endoplasmatic reticulum and the reduction in glycogen amount induced by PTH might be related to an enhancement of hepatic metabolism promoted by the hormone / Doutorado / Histologia / Doutor em Biologia Buco-Dental

Indiana University-Purdue University Indianapolis (IUPUI) / The purpose of this study was to investigate the possibility of accomplishing simultaneous intrusion and distal root movement of monkey premolar teeth utilizing the molar teeth as anchorage. Four Macaque speciosa monkeys were used as the experimental specimens. Root springs of the type used in the segmented arch technique were used to apply unequal moments to the premolar and molar teeth. The root movement was accomplished by a couple generated by the unequal moments. The results were analyzed cephalometrically and histologically. It was found that it is possible to accomplish distal root movement and intrusion of the monkey premolars when the anchorage is restrained from eruption. When the anchorage is not restrained intrusion is minimal. Root resorption was found to be associated with the magnitude of the difference in the moments applied and the amount of tooth movement.

Indiana University-Purdue University Indianapolis (IUPUI) / It has been proposed that jaw deformation during function may be a contributor to supraosseous tooth eruption. This could be through a transient pressure gradient in the flexed bone and/or tension in the PDL fibers produced by socket deformation. Thus, the purpose of this study was to determine if forces applied to a fresh frozen canine mandible can lead to occlusal displacement of a tooth. Seven hemisected canine mandibles (14 specimens) were used to test this hypothesis. A force cycle (10 N to 100 N to 300 N) was applied by a Bionix testing machine (MTS TM Systems Corp., Minneapolis, Minn.) to the mandible in three point bending. Digital radiographs were taken at each change in force level using computer digitized radiography (CDR, TM Schick Technologies, Inc., Long Island City, N.Y.). By measuring the relative movement of metal markers (1 mm steel balls) placed into the mandible and the crown of the 2nd premolar, the amount of tooth eruption was calculated. With cyanoacrylate cement (Archer® Instant Bonding Adhesive, Tandy Corp, Ft. Worth, Texas) injected into the PDL space of the same tooth, the test was repeated one week later. These "ankylosed" teeth served as controls. The eruption distances were compared between the experimental and the ankylosed teeth by means of repeated measures analysis of variance. The only significant occlusal displacement was noted as the force was initially raised to 100 N (21.7 ± 40.6 μm). Due to the possible breakdown of the cyanoacrylate cement, the ankylosed teeth also showed evidence of eruption with the continued application of force. Although significant eruption was noted, the displacements were very small. PDL degradation, viscoelastic behavior, measurement of small displacements, limited resolution radiography, and two dimensional analysis are contributing factors to the uncertainty of the results. Further investigation is required to determine if jaw deformation during function is a viable mechanism leading to the occlusal displacement of teeth.

Thesis (M.S.)--University of Michigan, 1989.

Thesis (M.S.)--University of Michigan, 1989.

Thesis (M.S.)--University of Michigan, 1960.

Thesis--Rijksuniversiteit te Leiden, 1951.

Thesis--Rijksuniversiteit te Leiden, 1951.

Proposição: avaliar radiograficamente e histologicamente o comportamento do incisivo inferior de rato frente à realização de uma solução de continuidade em sua porção radicular mediana quanto ao comportamento do tecido pulpar e dos enxertos ósseos alógenos congelados à -80°C e a evolução do seu processo eruptivo dentário em alvéolos quando expostos aos efeitos da ação do ultra-som. Metodologia: estudo experimental in vivo, amostragem randomizada, com um grupo teste e outro controle. Constou de 29 ratos, da espécie Rattus novergicus albinus, linhagem Wistar, machos, divididos em quatro grupos, correspondendo aos tempos de sete, 14, 21 e 45 dias. Em cada grupo, cinco animais foram teste e dois animais foram controle. Em cada animal foi removido, cirurgicamente, o segmento medial do incisivo inferior direito. Entretanto, nos animais do grupo experimental foram realizados enxertos de osso alógeno congelado a -80°C, nos 2,0mm distais do total da cavidade alveolar cirurgicamente obtida com ultra-som.Resultados: aos sete dias observou-se estrutura tecidual desorganizada e ricamente vascularizada em ambos os grupos avaliados e início da integração do enxerto ósseo no grupo teste. Aos 14 dias seguem as mesmas observações descritas aos sete dias com progressão da incorporação do enxerto ósseo. Aos 21 dias, observa-se intensa neoformação óssea, ricamente vascularizada em ambos grupos. Aos 45 dias, existe também uma intensa neoformação óssea alveolar, fechando o alvéolo e a completa integração dos enxertos ósseos. Em todos os grupos observou-se que houve mínimo crescimento do segmento dentário proximal e que o mesmo manteve sua vitalidade pulpar. Conclusão: Após a realização deste estudo foi possível concluir que: o tecido pulpar, contido no segmento proximal manteve sua vitalidade, reagindo na forma de reparação dentinária compatível com o dente humano; o enxerto ósseo alógeno congelado evolui favoravelmente no processo de incorporação a partir de um leito receptor criado no alvéolo dentário e que o incisivo inferior do rato, submetido à odontossecção com ultra-som não seguiu com o processo de crescimento e erupção em seu segmento proximal, podendo estar relacionado com o reparo do alvéolo devido à intensa neoformação óssea, provavelmente por efeito do ultra-som. / Proposition: This study aimed to evaluate, both radiographically and histologically, the behaviour of a rat’s inferior incisive concerning the realization of a continuous solution in its medial radicular portion, regarding the behaviour of the pulpal tissue and allogeneic freezing bone grafts, at –80° C, as well as the evolution in its dental eruptive process in alveoli exposed to ultrasound effects. Methodology: An in vivo experimental study, using randomized samples, including a study group and a control group. Twenty-nine Wistar strain male rats, Rattus novergicus albinus species, divided into 4 groups, corresponding to 7, 14, 21, and 45 days. From each group, five animals were considered as the test group, and two of them as the control group. Each animal had the medial segment of the right inferior incisive surgically removed. However, the animals from the test group underwent allogeneic freezing bone grafts, at –80° C, in a range of 2.0 mm distal of the total alveolar cavity, surgically performed by ultrasound. Results: In seven days a disorganized and well-vascularized tissue structure in both assessment groups as well as the beginning of the integration of the bone graft in the experimental group could be observed. In 14 days similar observations to day 7 and progression in the incorporation of the bone graft were found. In 21 days intense, well-vascularized, bone neoformation was observed in both groups. In 45 days an intense neoformation of the alveolar bone could also be observed, with the closure of the alveolus, and the complete integration of the bone grafts. All groups had a minimum growth of the dental proximal segment, which maintained its pulpal vitality. Conclusion: The study concluded that the pulpal tissue contained in the proximal segment maintained its vitality, providing a reaction compatible with human tooth regarding dental repairing. The allogeneic freezing bone graft evolved favorably in the incorporation process from a receiver layer created inside the dental alveolus. It was also concluded the rat’s inferior incisive which underwent ultrasonic dental cutting did not follow the growth and eruption process in its proximal segment, what might be related to the alveolus repair due to intense bone neoformation, probably caused by the ultrasound effect.

Proposição: avaliar radiograficamente e histologicamente o comportamento do incisivo inferior de rato frente à realização de uma solução de continuidade em sua porção radicular mediana quanto ao comportamento do tecido pulpar e dos enxertos ósseos alógenos congelados à -80°C e a evolução do seu processo eruptivo dentário em alvéolos quando expostos aos efeitos da ação do ultra-som. Metodologia: estudo experimental in vivo, amostragem randomizada, com um grupo teste e outro controle. Constou de 29 ratos, da espécie Rattus novergicus albinus, linhagem Wistar, machos, divididos em quatro grupos, correspondendo aos tempos de sete, 14, 21 e 45 dias. Em cada grupo, cinco animais foram teste e dois animais foram controle. Em cada animal foi removido, cirurgicamente, o segmento medial do incisivo inferior direito. Entretanto, nos animais do grupo experimental foram realizados enxertos de osso alógeno congelado a -80°C, nos 2,0mm distais do total da cavidade alveolar cirurgicamente obtida com ultra-som.Resultados: aos sete dias observou-se estrutura tecidual desorganizada e ricamente vascularizada em ambos os grupos avaliados e início da integração do enxerto ósseo no grupo teste. Aos 14 dias seguem as mesmas observações descritas aos sete dias com progressão da incorporação do enxerto ósseo. Aos 21 dias, observa-se intensa neoformação óssea, ricamente vascularizada em ambos grupos. Aos 45 dias, existe também uma intensa neoformação óssea alveolar, fechando o alvéolo e a completa integração dos enxertos ósseos. Em todos os grupos observou-se que houve mínimo crescimento do segmento dentário proximal e que o mesmo manteve sua vitalidade pulpar. Conclusão: Após a realização deste estudo foi possível concluir que: o tecido pulpar, contido no segmento proximal manteve sua vitalidade, reagindo na forma de reparação dentinária compatível com o dente humano; o enxerto ósseo alógeno congelado evolui favoravelmente no processo de incorporação a partir de um leito receptor criado no alvéolo dentário e que o incisivo inferior do rato, submetido à odontossecção com ultra-som não seguiu com o processo de crescimento e erupção em seu segmento proximal, podendo estar relacionado com o reparo do alvéolo devido à intensa neoformação óssea, provavelmente por efeito do ultra-som. / Proposition: This study aimed to evaluate, both radiographically and histologically, the behaviour of a rat’s inferior incisive concerning the realization of a continuous solution in its medial radicular portion, regarding the behaviour of the pulpal tissue and allogeneic freezing bone grafts, at –80° C, as well as the evolution in its dental eruptive process in alveoli exposed to ultrasound effects. Methodology: An in vivo experimental study, using randomized samples, including a study group and a control group. Twenty-nine Wistar strain male rats, Rattus novergicus albinus species, divided into 4 groups, corresponding to 7, 14, 21, and 45 days. From each group, five animals were considered as the test group, and two of them as the control group. Each animal had the medial segment of the right inferior incisive surgically removed. However, the animals from the test group underwent allogeneic freezing bone grafts, at –80° C, in a range of 2.0 mm distal of the total alveolar cavity, surgically performed by ultrasound. Results: In seven days a disorganized and well-vascularized tissue structure in both assessment groups as well as the beginning of the integration of the bone graft in the experimental group could be observed. In 14 days similar observations to day 7 and progression in the incorporation of the bone graft were found. In 21 days intense, well-vascularized, bone neoformation was observed in both groups. In 45 days an intense neoformation of the alveolar bone could also be observed, with the closure of the alveolus, and the complete integration of the bone grafts. All groups had a minimum growth of the dental proximal segment, which maintained its pulpal vitality. Conclusion: The study concluded that the pulpal tissue contained in the proximal segment maintained its vitality, providing a reaction compatible with human tooth regarding dental repairing. The allogeneic freezing bone graft evolved favorably in the incorporation process from a receiver layer created inside the dental alveolus. It was also concluded the rat’s inferior incisive which underwent ultrasonic dental cutting did not follow the growth and eruption process in its proximal segment, what might be related to the alveolus repair due to intense bone neoformation, probably caused by the ultrasound effect.