Glycine-arginine-alpha-ketoisocaproic acid calcium (GAKIC) is an amino acid combination postulated to improve dynamic performance of skeletal muscle during acute, anaerobic exercise in healthy individuals. Purpose: The purpose of this study was to determine the ergogenic effects of GAKIC ingestion on resistance training performance in both trained male and female participants. Methods: Utilizing a double-blinded, crossover design, male participants completed a lower body leg press resistance exercise protocol and female participants completed a lower body leg extension resistance exercise protocol once using 10.2 g GAKIC and the other with a placebo. Results: A significant increase in TLV after GAKIC supplementation was observed in both male and female participants performing a lower body resistance exercise. No significant differences were found in lower body 1RM, HR, BLa, and Glucose between conditions in both groups. Conclusion: We concluded with the specific exercise protocols that were implemented, GAKIC increased TLV in the lower body exercises.