According to ¡§The 2003 e-learning readiness rankings report¡¨, published by The Economist and IBM in 2004, Taiwan was ranked the third place of e-learning readiness in Asia. In 2002, Taiwan¡¦s government set up the goal to establish the high quality e-learning environment and became one of the leading countries of doing research in e-learning technology. This research adopts the model of training transfer which includes four independent variables¡Ðtrainee characteristics, training contents, system design, and working environment, one intervening variable¡Ðlearning satisfaction, and one dependent variable¡Ðtraining transfer effectiveness. Through questionnaire investigation and data analysis, the conclusions are in the following¡G 1. When the trainee¡¦s motive is higher and easily accept computer training, trainee¡¦s satisfaction and training transfer effectiveness is higher. 2. When the trainee adopts proper training time or course and interacts well with other trainees, trainee¡¦s satisfaction and training transfer effectiveness is higher. 3. When the working environment is beneficial to training, trainee¡¦s satisfaction and training transfer effectiveness is higher. 4. When the e-learning system is easier to use and provides various evaluation ways, trainee¡¦s satisfaction and training transfer effectiveness is higher. 5. The intervening effect of learning satisfaction between trainee characteristics, training contents, system design, working environment, respectively, and training transfer effectiveness obviously existed.