Global ETD Search

1	Macromolecular Interactions in West Nile Virus RNA-TIAR Protein Complexes and of Membrane Associated Kv Channel Peptides Zhang, Jin 01 July 2013 (has links) Macromolecular interactions play very important roles in regulation of all levels of biological processes. Aberrant macromolecular interactions often result in diseases. By applying a combination of spectroscopy, calorimetry, computation and other techniques, the protein-protein interactions in the system of the Shaw2 Kv channel and the protein-RNA interactions in West Nile virus RNA-cellular protein TIAR complex were explored. In the former system, the results shed light on the local structures of the key channel components and their potential interaction mediated by butanol, a general anesthetic. In the later studies, the binding modes of TIAR RRM2 to oligoU RNAs and West Nile virus RNAs were investigated. These findings provided insights into the basis of the specific cellular protein–viral RNA interaction and preliminary data for the development of strategies on how to interfere with virus replication Macromolecular interaction Thermodynamics tran-cis Isomerization Exchange spectroscopy Shaw2 Kv channel S4-S5 linker S6 Anesthetics DPC micelles Paramagnetic relaxation enhancement West Nile virus RRM2 Binding interactions Molecular docking Isothermal titration calorimetry Adenine H2 Long-range coupling