To assess a wide range of stressors and traumas, we developed a trauma and stressors exposure checklist for adults to capture a wide variety of potential trauma exposure. This study details the extensive development and validation process of the Comprehensive Trauma and Stressors Checklist (CTSC). Study 1 consisted of item pool generation, review of existing trauma measures, content analysis, and expert review and input. Study 2 analyzed reliability and validity of the updated checklist. Participants were recruited from college and community samples (N = 200) and completed the CTSC along with five additional measures. The CTSC demonstrated high internal consistency (α =.96). Good temporal stability was established with a subset of participants (n = 28) who completed the survey twice for test-retest reliability (r = 0.86, p < .001). Significant correlations between scores on the CTSC and the Stressful Life Events Screening Questionnaire, Life Events Checklist for the DSM-5, Intersectional Discrimination Index, and Spiritual Abuse Questionnaire are evidence of strong convergent validity. Finally, the lack of significant correlations between CTSC scores and theoretically unrelated constructs supported the discriminant validity of the CTSC. This study provides preliminary evidence that the CTSC is a reliable and valid measure of exposure to traumas and stressors. Future studies with the CTSC will examine measurement invariance, exploratory and confirmatory factor analysis, and reading level.