This dissertation thesis deals with elastohydrodynamic lubrication (EHL) of surfaces with real roughness. Systematic study of surface harmonic components behaviour, during their passage of the EHL contact, by other authors led to retrieval of a uniform principle according which individual harmonic components deform inside the contact. Although the amplitude attenuation model can provide rapid tool for more accurate surface assessment in design process it has not been validated on real surfaces till now. The thesis represents pioneering work on quantitative analysis of real surfaces EHD measurements. It contains original and new results with real and model roughness features that in many cases confirm theoretical expectations. Deeper understanding of surface roughness behaviour inside EHL contact, which is typical for machine parts like bearings, gears, cams etc., can help to improve design process of the components and ultimately enable machines and equipment to work with lower energy requirements and higher durability.