Thesis (Ph. D.)--University of Missouri--Columbia, 1998. / "December 1998" Typescript. Vita. Includes bibliographical references (l. 140-148). Also available on the Internet.

Thesis (Ph.D.)--University of Alberta, 2010. / A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Doctor of Philosophy, Centre of Neuroscience. Title from pdf file main screen (viewed on April 18, 2010). Includes bibliographical references.

Thesis (Ph. D.)--University of Virginia, 1998. / Energy coupling in the F₀F₁ ATP synthase. Includes bibliographical references (p. 160-178). Also available online through Digital Dissertations.

Thesis (Ph. D.)--Case Western Reserve University, 2006. / [School of Medicine] Department of Molecular Biology and Microbiology. Includes bibliographical references. Available online via OhioLINK's ETD Center.

Thesis (Ph.D. in Molecular Biology) -- University of Colorado, 2005. / Typescript. Includes bibliographical references (leaves 100-113). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;

Thesis (Ph. D.)--University of Florida, 2004. / Typescript. Title from title page of source document. Document formatted into pages; contains 258 pages. Includes vita. Includes bibliographical references.

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The human erythrocyte glucose transport protein (GLUT1) interacts with, and is regulated by, cytosolic ATP. This study asks the following questions concerning ATP modulation of GLUT1 mediated sugar transport. 1) Which region(s) of GLUT1 form the adenine nucleotide-binding domain? 2) What factors influence ATP modulation of sugar transport? 3) Is ATP interaction with GLUT1 sufficient for sugar transport regulation? The first question was addressed through peptide mapping, n-terminal sequencing, and alanine scanning mutagenesis of GLUT1 using [32P]-azidoATP, a photoactivatable ATP analog. We then used a combination of transport measurements and photolabeling strategies to examine how glycolytic intermediates, pH, and transporter oligomeric structure affect ATP regulation of sugar transport. Finally, GLUT1 was reconstituted into proteoliposomes to determine whether ATP is sufficient for the modulation of GLUT1 function in-vitro. This thesis presents data supporting the hypothesis that residues 332-335 contribute to the efficiency of adenine nucleotide binding to GLUT1. In addition, we show that AMP, acidification, and conversion of the transporter to its dimeric form antagonize ATP regulation of sugar transport. Finally, we present results that support the proposal that ATP interaction with GLUT1 is sufficient for transport modulation.

Magister Scientiae - MSc (Biotechnology) / Brassica napus is a plant that is used for human and animal consumption. This plant is also used for phytoremediation due to its relatively higher level of heavy metal tolerance. In South Africa, mining is one of the main drivers of the economy. One of the major negative environmental impacts of mining is heavy metal contamination. Soil metal content can rise to levels that are quite high and can even have a negative impact on the yields of B. napus crop. The glucosinolate-myrosinase system of B. napus is a system that is used as defence against biotic stressors. Indole glucosinolate breakdown products have been proven to enhance the antioxidant capacity of plants. Some have also shown growth promoting properties in plants. We studied the effect of exogenous DIM on B. napus and it role in Zr induced heavy metal stress. Germination percentages revealed that DIM increased germination, Zr application decreased germination and the DIMZr treatment reversed the negative impact of Zr application on B. napus. The effect of treatments on the biomass of B. napus was assessed by determining the dry weights. Results show that exogenous DIM improves biomass. Zr application decreased biomass and DIM-Zr treatment ameliorated the effect of Zr application. / 2020-08-31

Magister Scientiae - MSc (Medical BioSciences) / Central nervous system (CNS) infections are a therapeutic challenge. This is partly due to insufficient drug penetration across the blood-brain barrier (BBB). The BBB is a specialized, highly selective, metabolically active physiological barrier that regulates the movement of molecules into-and-out of the brain. As a result, large hydrophilic antibiotics such as colistin poorly penetrate to the CNS. Colistin is an old 'last line of defence'; a gram-negative antibiotic that has seen its clinical re-emergence due to the surge of multidrug resistance (MDR) infections. However, owing to systemic toxicity, increasing the intravenous dosage, in order to obtain higher CNS penetration, is inimical. Chitosan (CS) based nanoparticles (NPs) have been proposed as drug delivery systems across the BBB. CS is a cationic, natural polysaccharide that has the ability to be complexed with multivalent polymers like dextran (DS) thus forming CS-DS NPs. Naturally, CS has remarkable inherent features such as biocompatibility, biodegradability, ability to encapsulate poorly soluble drugs and it is favourable for endothelial cell uptake. However, polymeric NPs (even those derived from natural polysaccharides) have limited use due to toxicity. Considering the vital role of the BBB, toxicity would denote dire effects on CNS functioning. Therefore, treatment of CNS infections fringes on a deeper understanding of the interactions between drug delivery systems and the BBB.