Se realizó un estudio descriptivo, retrospectivo, transversal; la Unidad de muestreo fueron los pacientes con diagnostico clínico de Síndrome del Pronador sometidos a Liberación Quirúrgica en el Departamento de Ortopedia y Traumatología del Hospital Nacional Edgardo Rebagliati Martins entre Junio del 2003 y Mayo del 2004. Se realizó además la liberación quirúrgica del Ligamento Transverso del Carpo en todos los pacientes con cuadro clínico de compresión del Túnel del Carpo asociado al Síndrome del Pronador. El análisis estadístico se realizó con el programa SPSS Vs. 11. Fueron seleccionados 26 casos, se encontró que 73 % (19) eran mayores de 45 años y el 69.2% (18) de sexo femenino, el 76.9% (20) se dedicaban a labores que se relacionaban a actividades manuales, el 88.5 % (23) eran diestros y en el 65.4 % (17) el atrapamiento nervioso se encontró en la extremidad derecha, en un 84.6 % (22 ) se realizó una liberación del Síndrome del Túnel del Carpo homolateral. Respecto de las maniobras clínicas, el 46.2% (12) presentaron dolor en el punto pronador, seguido de un 23.1 % (06) supinación contra resistencia, y un 23.1% (06) pronación contra resistencia; los hallazgos quirúrgicos indicaron que en el 42.3 % (11) fue la aponeurosis bicipital, en un 30.8 % (08) la banda del pronador redondo y en un 19.2 % (05) causas tumorales las responsables de la compresión. Las complicaciones postoperatorias, fueron hematoma 30.8 % ( 08 ), e infección en el 3.8 % ( 01) de los casos. / It was carried out a descriptive, retrospective, transverse study; the sampling Unit was the patients with diagnose clinical of the Pronator Syndrome to Surgical Liberation in the Department of Orthopedics and Traumatology of the National Edgardo Rebagliati Martins Hospital between June of the 2003 and May of the 2004. It was also carried out the surgical liberation of the Transverse Ligament of the Carpo in all the patients with clinical square of compression of the Tunnel of the Carpo associated to the Pronator Syndrome. The statistical analysis was carried out with the program SPSS Vs. 11. 26 cases were selected, it was found that 73% (19) were bigger than 45 years and 69.2% (18) of female sex, 76.9% (20) were devoted to works that they were related to manual activities, 88.5% (23) were skillful and in 65.4% (17) the nervous atrapamiento was in the right extremity, in 84.6% (22) was carried out a liberation of the Syndrome of the Tunnel of the Carpo homolateral. Regarding the clinical maneuvers, 46.2% (12) presented pain in the point pronador, followed by 23.1% (06) supination against resistance, and 23.1% (06) pronation against resistance; the surgical discoveries indicated that in 42.3 % (11) was the aponeurosis bicipital, in 30.8 % (08) the band of the round pronador and in 19.2 % (05) cause tumorales those responsible for the compression, the postoperative complications, were hematoma 30.8 % (08), and infection in 3.8 % (01) of the cases.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

La función motora y sensitiva de la mano es la herramienta básica principal en el ejercicio de la odontología, toda actividad clínica odontológica necesita el adecuado funcionamiento de la mano para realizar una labor eficiente. El Síndrome del Túnel Carpal es un desorden producido por una compresión nerviosa, que tiene entre sus principales factores de riesgo muchas de las actividades o ejercicios manuales a los que un odontólogo se somete en su ejercicio común. Este síndrome ha sido estudiado anteriormente en países extranjeros donde se ha encontrado en muchos casos mayor prevalencia en la comunidad odontológica sea de profesionales, estudiantes, higienistas dentales y/o asistentes dentales que en la población general. Es, por lo tanto, de relevante importancia el reconocimiento y establecimiento de su sintomatología en nuestra realidad, por lo que la presente investigación se sitúa entre los primeros estudios basales que sobre esta enfermedad ocupacional se realizan en nuestro país. / Tesis

Se realizó un estudio descriptivo, retrospectivo, transversal; la Unidad de muestreo fueron los pacientes con diagnostico clínico de Síndrome del Pronador sometidos a Liberación Quirúrgica en el Departamento de Ortopedia y Traumatología del Hospital Nacional Edgardo Rebagliati Martins entre Junio del 2003 y Mayo del 2004. Se realizó además la liberación quirúrgica del Ligamento Transverso del Carpo en todos los pacientes con cuadro clínico de compresión del Túnel del Carpo asociado al Síndrome del Pronador. El análisis estadístico se realizó con el programa SPSS Vs. 11. Fueron seleccionados 26 casos, se encontró que 73 % (19) eran mayores de 45 años y el 69.2% (18) de sexo femenino, el 76.9% (20) se dedicaban a labores que se relacionaban a actividades manuales, el 88.5 % (23) eran diestros y en el 65.4 % (17) el atrapamiento nervioso se encontró en la extremidad derecha, en un 84.6 % (22 ) se realizó una liberación del Síndrome del Túnel del Carpo homolateral. Respecto de las maniobras clínicas, el 46.2% (12) presentaron dolor en el punto pronador, seguido de un 23.1 % (06) supinación contra resistencia, y un 23.1% (06) pronación contra resistencia; los hallazgos quirúrgicos indicaron que en el 42.3 % (11) fue la aponeurosis bicipital, en un 30.8 % (08) la banda del pronador redondo y en un 19.2 % (05) causas tumorales las responsables de la compresión. Las complicaciones postoperatorias, fueron hematoma 30.8 % ( 08 ), e infección en el 3.8 % ( 01) de los casos. / It was carried out a descriptive, retrospective, transverse study; the sampling Unit was the patients with diagnose clinical of the Pronator Syndrome to Surgical Liberation in the Department of Orthopedics and Traumatology of the National Edgardo Rebagliati Martins Hospital between June of the 2003 and May of the 2004. It was also carried out the surgical liberation of the Transverse Ligament of the Carpo in all the patients with clinical square of compression of the Tunnel of the Carpo associated to the Pronator Syndrome. The statistical analysis was carried out with the program SPSS Vs. 11. 26 cases were selected, it was found that 73% (19) were bigger than 45 years and 69.2% (18) of female sex, 76.9% (20) were devoted to works that they were related to manual activities, 88.5% (23) were skillful and in 65.4% (17) the nervous atrapamiento was in the right extremity, in 84.6% (22) was carried out a liberation of the Syndrome of the Tunnel of the Carpo homolateral. Regarding the clinical maneuvers, 46.2% (12) presented pain in the point pronador, followed by 23.1% (06) supination against resistance, and 23.1% (06) pronation against resistance; the surgical discoveries indicated that in 42.3 % (11) was the aponeurosis bicipital, in 30.8 % (08) the band of the round pronador and in 19.2 % (05) cause tumorales those responsible for the compression, the postoperative complications, were hematoma 30.8 % (08), and infection in 3.8 % (01) of the cases.

No description available.

Cryopreservation of oocytes is a challenge. Studies were conducted to vitrify mouse zygotes and cumulus-intact bovine oocytes from follicles of different diameters, small (≤ 4 mm) and medium (4 to 10 mm), using nylon mesh. The specific goals were to assess changes in apoptotic gene expression (Fas-FasL, Bax, Bcl-2, and survivin) in conjunction with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and caspase assays. Mouse zygotes were exposed to increasing concentrations of ethylene glycol (EG), Ficoll-70 and sucrose in phosphate buffered saline (PBS) for vitrification on nylon mesh and plunged into liquid nitrogen. Warming resulted in 81.7% morphological survival. The rate of blastocyst formation was 59.9% for vitrified zygotes but, this was significantly lower than that of non vitrified embryos (66.2%). There was no difference in the hatching rates between groups. Both Fas and FasL mRNA were detected at the 4-cell and morula stages, suggesting Fas signaling was operational in early embryos. The level of expression of Bax mRNA tended to increase, while expression of survivin mRNA was not different for 2- and 4-cell embryos. Fragmented embryos showed an increase in Bax mRNA levels, while survivin mRNA level was reduced. In the second experiment, vitrification of bovine oocytes was carried out. Pre-cooled cryovials resulted in 98.9% morphological survival. The oocytes from small and medium size follicles had a significant impact on cleavage (53.7 ± 1.6% vs. 43.8 ± 1.6%, respectively) and blastocyst rates were 16.9 ± 1.0% and 11 ± 1.2%, respectively. Follicle size for oocytes had no impact on the expression of apoptotic genes. The Fas-FasL and Bax-Bcl-2 mRNA showed increased expression after vitrification, but tended to decrease after 9 h of maturation. Yet, results from TUNEL and caspase assays did not support the evidence of the downstream apoptotic signaling pathway in embryos. The semen utilized for in vitro fertilization in both vitrified and control oocytes responded differently in the 4 tested bulls than their field fertility record. The altered transcriptional activities of apoptotic genes, Fas-FasL and Bax-Bcl-2, and survivin were indicative of possible apoptotic activity in vitrified embryos and oocytes subjected to in vitro fertilization. / Ph. D.

Disertační práce představuje aplikaci hypoplastického konstitučního modelu při realizaci NRTM tunelu v prostředí městské zástavby s cílem obdržet "class A" predikce chování zeminového masivu. Tunely Královo Pole tvoří hlavní konstrukci severní části Velkého městského okruhu v Brně. Tunely o šířce 14 m a výšce 12 m jsou raženy v prostředí tuhých brněnských jílů s výškou nadloží v rozmezí 6 až 21 metrů. Materiálové parametry hypoplastického modelu kalibrované na výsledky laboratorních experimentů byly optimalizovány využitím dat geotechnického monitoringu a následně použity při simulacích tunelu. Po průchodu ražby profilu tunelu zvoleným úsekem staničení došlo k vyhodnocení prediktivních schopností a vhodnosti konstitučního modelu. Druhým konstitučním modelem použitým při simulacích průzkumné štoly a plain strain analýze tunelu je Mohr - Coulombův model. Metoda redukce napětí využitá v procesu optimalizace parametrů byla vyhodnocena formou porovnání s 3D simulacemi. K vyhodnocení posloužily simulace třech skutečných podzemních konstrukcí s nízkým nadložím realizovaných v prostředí tuhých jílů. Abstract The thesis demonstrates the application of a hypoplastic model in class A predictions of a NATM tunnel in an urban environment. The Kralovo Pole tunnel is a main construction of a northern part of a...

No description available.

Eukaryotic translation initiation factor 5A (eIF-5A) is the only known cellular protein that contains the post-translationally derived amino acid, hypusine. Initially, eIF-5A was named as a translation initiation factor because of its capability to stimulate the formation of methionyl-puromycin, which mimics the first peptide bond formation during protein synthesis, under <em>in vitro</em> conditions. Subsequently, however, this proposed function of eIF-5A has been questioned because a similar effect on translation was not observed <em>in situ</em>. Moreover, eIF-5A appears not to be required for general protein synthesis. Rather, there is evidence that it facilitates the translation of specific subsets of mRNAs required for cell proliferation as well as apoptosis. <br /><br /> There are two isoforms of eIF-5A in the human genome which have designated eIF-5A1 and eIF-5A2. The objective of the present study was to gain an increased understanding of the roles of eIF-5A1 and eIF-5A2 during apoptosis and cell proliferation using human cell lines as a model system. Apoptosis was induced by treating the cells with Actinomycin D or sodium nitroprusside (SNP), which initiate programmed cell death by different mechanisms. It was observed for both normal and cancer cells that eIF-5A1 protein is up-regulated during apoptosis induced by Actinomycin D or SNP, whereas eIF-5A1 mRNA is constitutively expressed and does not change in abundance during this treatment. The up regulation of eIF-5A1 protein levels in the absence of a corresponding up-regulation in eIF-5A1 mRNA suggests that eIF-5A1 may be post-transcriptionally regulated. Moreover, eIF-5A1 protein up-regulation was stronger in normal cells than in cancer cells. By contrast, eIF-5A2 protein was below detection levels during apoptosis in both normal and cancer cells, although the corresponding transcript was detectable by semi-quantitative RT-PCR. This is attributable to inefficient translation of eIF-5A2 mRNA. <br /><br /> The effects of eIF-5A1 and eIF-5A2 on cell proliferation were examined by modulating the levels of serum in cultures of UACC-1598 cells, which are ovarian cancer cells that express high levels of both isoforms of eIF-5A. Serum starvation, which induces cell cycle arrest and ensuing apoptosis, followed by the re-addition of serum had no effect on the transcript levels of either eIF-5A1 or eIF-5A2. However, eIF-5A1 and eIF-5A2 proteins were both up-regulated within 24 hours of the initiation of serum starvation, and this coincided temporally with the onset of apoptosis as measured by TUNEL and a subsequent decline in viable cells. <br /><br /> The data indicate that eIF-5A1 and eIF-5A2 are both post-transcriptionally regulated and that they have functionally redundant roles in apoptosis.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.