A utilização da eletroestimulação muscular é uma estratégia utilizada para investigação da fadiga neuromuscular. Entretanto, esta técnica é pouco aplicada na musculatura extensora do cotovelo em função de limitações biomecâncias (atuação de músculos sinergistas), neurais (influência de reflexo neural) e fisiológicas (facilidade em atingir estados de potencialização pós-ativação e fadiga). Portanto, o objetivo deste estudo foi padronizar procedimentos para avaliação neuromuscular da musculatura extensora do cotovelo. Para isso, foram realizados três estudos que envolveram a adequação da técnica de eletroestimulação e um \"estudo principal\" para validar um novo protocolo de avaliação neuromuscular. Após resultados positivos em estudos complementares, a presente dissertação propôs a validação de um protocolo para análise neuromuscular com contrações submáximas para a musculatura extensora do cotovelo. Vinte participantes com idade de 25,1 ± 6,8 anos foram submetidos, em dias diferentes, a dois esforços, um a fim de promover fadiga central e fadiga periférica respectivamente. Antes e após os esforços envolvendo fadiga (periférica ou central) foram realizadas avaliações de fadiga neuromuscular para musculatura extensora do joelho (protocolo já validado) e musculatura extensora do cotovelo (protocolo proposto). Verificou-se que: (i.) o modelo de análise proposto não promove nenhum tipo de fadiga ou potencialização pós-ativação; (ii) a resposta de um pulso controle no início e no fim do modelo de análise proposto promovem respostas diferentes; (iii) o modelo proposto foi capaz de identificar redução da resposta neuromuscular; (iv) o esforço envolvendo fadiga central não promoveu fadiga central significante; (v) a utilização de um modelo matemático para investigação de fadiga neuromuscular (coeficientes resultantes de uma regressão linear) com os valores de estimulação em contrações submáximas foi sensível a fadiga mas não foi capaz de discriminar fadiga central e periférica. É possível concluir que o modelo de análise proposto para a musculatura extensora do cotovelo apresentou sensibilidade para verificar a fadiga neuromuscular. Entretanto, este não foi capaz de discriminar fadiga central e periférica. A utilização da regressão linear parece ser uma boa estratégia para diferenciar os dois modelos de fadiga e deve ser estudada com mais profundidade futuramente. / Muscular electrostimulation is a strategy used to investigate neuromuscular fatigue. However, this technique is poorly applied in the elbow extensor muscles due to the limitations of biomechanics (acting of synergistic muscles), neural (influence of neural reflex) and physiological (easiness to achieve post-activation and fatigue states of potentiation). Therefore, the purpose of this study was to standardize a neuromuscular evaluation strategy for the elbow extensor musculature. Thus, three complementary studies aimed at adjusting the electrostimulation technique and one \"main study\" to validate a new neuromuscular assessment protocol were realized. After positive results in complementary studies, the present dissertation proposed a protocol validation for neuromuscular analysis with submaximal contractions on the elbow extensor musculature. Twenty participants with 25.1 ± 6.8 years old performed, on different days, two efforts, one to promote central fatigue and peripheral fatigue respectively. Before and after the exercise involving fatigue (peripheral or central) there were realized neuromuscular fatigue evaluations for knee extensor muscles (protocol already validated) and elbow extensor musculature (protocol). The results indicate that: (i) the proposed analysis model does not promote any type of fatigue or post-activation potentiation; (ii) the response of a control pulse at the beginning and end of the proposed analysis model promotes different responses; (iii) the proposed model was able to identify reduction of neuromuscular response; (iv) the effort involving central fatigue did not promote significant central fatigue; (v) the use of a mathematical model to investigate neuromuscular fatigue (coefficients resulting from a linear regression) with the values of stimulation in submaximal contractions was sensitive after fatigue but was not able to discriminate central and peripheral fatigue. It is possible to conclude that the proposed analysis model for the elbow extensor musculature presented sensitivity to verify the neuromuscular fatigue. However, it was not able to discriminate central and peripheral fatigue. The use of linear regression seems to be a good strategy to differentiate the two models of fatigue and should be studied in more depth in the future.

Background: Registering a true maximum bite force on the most commonly-used force transducers is problematic. It is often believed that this is related mainly to discomfort and the fear of breaking teeth. Objectives: The aim of the project was to compare the suitability of different bite force measuring transducers including ones which were designed to improve subject comfort. The transducers used were a traditional strain-gauge transducer with and without covering with ethylene vinyl acetate (EVA) sheets, and a newly-developed pressure transducer. Methods: Five separate studies were performed in this project. The experiments were carried out on human volunteer subjects (aged 24 to 41 years). They were all dentate with no missing anterior teeth and with no crowns on these teeth. The following procedures were used in some or all of the studies: measurement of MVBF, electrical stimulation of the masseter muscle, and EMG recording from two pairs of jaw closing muscles. Results: The highest MVBF values were recorded on the pressure transducer, mean (± S.D.) 464 N ± 224 N; followed by the strain-gauge transducer with EVA sheets, 243 ± 80 N; and last of all the strain-gauge transducer with silicone indices, 165 ± 35 N; or acrylic indices, 163 ± 82 N. Significantly higher maximum potential bite forces were predicted by twitch interpolation for the pressure transducer (730 ± 199 N) than for the strain-gauge transducer with EVA sheets, 354 ± 67 N (Paired t test, P < 0.05). Significantly higher EMGs of the masseter and anterior temporalis muscles were found to be associated with MVBFs on the pressure transducer than with MVBFs on the strain-gauge transducer with EVA sheets (Paired t test, P < 0.05). Conclusions: It is concluded that: a) the pressure transducer system and to a lesser extent the strain-gauge transducer covered with EVA sheets seemed to overcome the fear associated with biting on the hard surfaces of the strain-gauge transducer alone; b) the pressure transducer may have some multi-directional capabilities which allow for total bite forces, or at least larger parts of them, to be recorded than on a uni-directional strain-gauge transducer.

The research presented in this thesis examined the issue of the compatibility of strength and endurance training within one training regime, termed concurrent training, in recreational cyclists. Various research designs used in the previous literature resulted in inconclusive findings. The overall aim of this thesis was therefore to examine, in three systematically designed studies, the effects of various components of concurrent training regimes on cycling efficiency and recovery, and to identify some of the mechanisms that may be responsible for the interference or impedance of strength and/or endurance adaptations.

The research presented in this thesis examined the issue of the compatibility of strength and endurance training within one training regime, termed concurrent training, in recreational cyclists. Various research designs used in the previous literature resulted in inconclusive findings. The overall aim of this thesis was therefore to examine, in three systematically designed studies, the effects of various components of concurrent training regimes on cycling efficiency and recovery, and to identify some of the mechanisms that may be responsible for the interference or impedance of strength and/or endurance adaptations.