<p>Mechanical oscillating drills and saws are used in orthopaedic surgery to cut bone and develop screw-holes; however, their use causes friction resulting in significant thermal damage. Ultrashort pulsed lasers appear well-suited to replace traditional tools as they have the ability to efficiently remove bone tissue while causing only minimal collateral damage. Laser ablation also has the added advantages of: (i) no mechanical vibration; (ii) minimal invasiveness; and (iii) small focus spot size. In this thesis work, we experimentally investigated a few key aspects of ultrashort laser ablation of bone tissue.</p> <p>The ablation threshold of unaltered bone was measured using the <em>D</em>²technique and found to range from 1.66 J/cm²± 0.87 J/cm² to 2.37 J/cm²± 0.78 J/cm² depending on incident pulse number. The reduction in ablation threshold with pulse number was an indication of an incubation effect. Using a power law model, the incubation coefficient, ζ, was measured to be 0.89 ± 0.03.</p> <p>The effect of specific laser parameters and drilling protocols on ablation efficiency was also characterized. For ultrashort pulses (≤10 ps), the removal rate was found to be inversely related to the pulse duration; however, irradiation with 5-10 ps pulses were also shown to result in significant tissue removal. With a pulse repetition rate of 1 kHz, the removal rate was observed to be highest when ablating with 50-100 pulses per spot.</p> <p>Larger volumes (>1 mm³) of bone tissue were removed using laser scanning procedures. A series of scanned concentric circles produced a structure ~2.4 mm deep; however, ablated side-lobes were present at oblique angles to the incident beam. A two-layer structure subsequently produced no side-lobes. The ablative precision in trabecular bone was observed to be less than cortical bone. Using mimicked Nd:YAG laser parameters, cylindrical drilling produced craters significantly less deep than those achieved with a typical Ti:Sapphire configuration. The ability to drill large-scale holes using low average pulse energies and optimized scanning procedures will alleviate the stringent requirements for optical components in clinical practice.</p> / Master of Science (MSc)