The Lake Victoria Basin (LVB) is endowed with natural resources which are drivers for sustainable development at local, national and regional levels. However, these resources are adversely affected by dynamic social, economic, environmental and political factors. Poor coordination and ineffective knowledge management programmes among various players within the LVB has led to duplication of efforts as substantial information and knowledge generated is unavailable to users. As a result, over-exploitation of natural resources has resulted in unsustainable development of the LVB. This study sought to establish the role of knowledge management in promoting sustainable development of the LVB. Specific objectives were to: determine the role of knowledge management in sustainable development of LVB; determine challenges and barriers hindering effective knowledge management; establish tools and technology that can be used to enhance knowledge management; and establish the strategies used by organisations in LVB to manage knowledge generated for sustainable development. A descriptive survey design was used in this study targeting 98 (with 76.5% response rate) officers of various ranks working in 26 organisations involved in development programmes/projects and located in the five partner states within the LVB. Data was collected using self administered questionnaires and a desk review. Findings showed most respondents agreeing that knowledge management is a systematic utilisation of policies, processes, activities and tools which empower organisations to apply knowledge to improve effectiveness, innovation and quality. Up to 93.9% of the organisations facilitated discovery, capture, storage and retrieval of knowledge. Most organisations encouraged documentation of lessons learnt, with some making it mandatory to deposit key documents in the library. Paper-based media was the most preferred knowledge storage format. Lack of an open-minded sharing environment, bureaucratic procedures and poor information systems were the common barriers to knowledge management, while lack of time (68.4%) was an obstacle to proper xvi documentation. The internet was the most popular knowledge management tool/technology; though technical issues were the most critical problems affecting ICT use (71.4%). Based on these findings, this study recommends a holistic and coordinated approach to knowledge management among all institutions working in the LVB to address the challenges of sustainable development in the basin. / Information Science / M. Inf. (Information Science)