This project investigated the physiological responses of grapevine to deficit irrigation strategies including partial rootzone drying (PRD) and regulated deficit irrigation (RDI). The principle objectives of the project were to (1) establish if the response to partial rootzone drying (PRD) is as a consequence of irrigation amount or a unique vine physiological response to PRD; (2) to investigate the effect of limiting environmental conditions on vine responses to PRD and deficit irrigation; (3) to investigate the effect of PRD and deficit irrigation on berry metabolism and maturation; and (4) effect of PRD and deficit irrigation on vine water-use. The experiment used field-grown Shiraz grapevines in a commercial vineyard in the Strathbogie Ranges in north-eastern Victoria. The experiment ran from season 2001/2002 through to 2003/2004 in a medium vigour, warm climate vineyard, with soils of low water holding capacity. (For complete abstract open document)

Water use efficiency (WUE), measured as the ratio of plant biomass to water consumption, is an essential agronomical trait for enhancing crop production under drought. Measuring water consumption is logistically difficult, especially in field conditions. The general objective of the present Thesis is to respond to three main questions: (i) can WUE be determined by using carbon isotope discrimination (CID), easy to measure?, (ii) how WUE and CID variation analysis can contribute to the genotypic selection of sunflower subjected to drought?, and (iii) can WUE variation be revealed by the variation of plant-water relation traits. Four experiments were carried out in greenhouse across two different years: (i) on two drought scenarios, progressive soil drying and stable water-stress, and (ii) on five levels of soil water content. The main traits that have been measured include WUE, CID, as well as plant-water relation traits, i.e. control of transpiration (FTSWt), water extraction capacity (TTSW), and dehydration tolerance (OA). A highly significant negative correlation was observed between WUE and CID, and a wide phenotypic variability was observed for both WUE and CID. A wide variability was also observed for FTSWt, TTSW and OA. The results provide new insight into the genetic control of WUE and CID related-traits, which, unlike to other crops, genetic control of WUE, CID, and TTSW in sunflower have never been reported in the literature. Further, quantitative trait loci (QTL) mapping for FTSWt was never reported in any plant species. The QTL for WUE and CID were identified across different drought scenarios. The QTL for CID is considered as a ‘‘constitutive’’ QTL, because it is consistently detected across different drought scenarios. The QTL for CID co-localized with the QTL for WUE, biomass and cumulative water transpired. Co-localization was also observed between the QTL for FTSWt and TTSW, between the QTL for TTSW and WUE-CID-biomass, as well as between the QTL for FTSWt-TTSW and biomass. This study highlights that WUE is physiologically and genetically associated with CID. CID is an excellent surrogate for WUE measurement, and can be used to improve WUE by using marker-assisted selection (MAS) to achieve the ultimate goal of plant breeding at genomic level.

Climate models have projected that arid and semiarid lands will experience warmer and drier conditions for the next 100 years. For the last twenty years, the Southwestern US has been experiencing one of the worst droughts over the last century, not only threatening ecological systems but also the water security of its population. Understanding the environmental processes that affect arid and semiarid forests are essential to better understand the water and carbon cycles, and tree-ring research has contributed valuable knowledge in this regard. There is a common understanding that moisture-stress has significant impacts on forested ecosystems and thereby on the global carbon and water cycles. Under persistent moisture deficit, a decline in growth, an increased proportion of wildfires, insect outbreaks, and mass-tree-mortality are often observed in arid and semi-arid forests, having large impacts on their carbon budgets and their capacity to act as a carbon sink. This study addresses the seasonal and regional climatic influences on the water-carbon relations in the ponderosa pine forests of the southwestern US (SW). This region is characterized by a complex climatology related to the North American Monsoon system (NAMS). A topic of interest in this dissertation is the role of the summer rainfall after the early-summer hyper-arid period in the region, providing a unique seasonal condition for these ecosystems to thrive. While these forests clearly rely on winter snowpack to drive much of their annual net primary productivity, the temporal and regional extent to which they supplement winter moisture with summer monsoon moisture needs to be clarified. The core of this dissertation is a study of the spatial and temporal variability of the stable carbon and oxygen isotopes in the cellulose of subsections of the tree rings (e.g., earlywood and latewood) collected from a network of thirteen sites along a latitudinal gradient extending from southern Arizona and New Mexico, through southwest Colorado, and up to northern Utah. The analysis is based on biological and physical processes and their close relationships with isotope effects to infer eco-physiological responses to climate variations over the last century. The stable carbon isotopes are used to derive intrinsic Water-Use Efficiency (iWUE) defined by the molar ratio of carbon gain to water loss. The stable oxygen isotope ratio is used to infer the variations on evaporative flux at the leaf level, which depend on stomatal conductance, atmospheric vapour pressure deficit at the leaf surface, and variations in the isotopic ratio of the source water. Both isotopic ratios are used to document variations in tree productivity and hydrologic vulnerability within the context of climate change impacts on this region. During the study, it was found that climate change in the SW has impacted the carbon and water cycles of these forests for at least the past twenty years. Additionally, seasonality influence the eco-physiology of ponderosa pine change along the latitudinal gradient, as shown by significant differences between EW and LW. These differences are explained by the large shifts in seasonal VPD, which are more evident in the southern part of our study region due to the mid-summer arrival of monsoon rains. These findings will be useful for regional natural resource managers and improves our understanding of seasonal influences on forest water–carbon relationships. This approach will also be useful to develop seasonally resolved paleoclimate and paleo-ecophysiological reconstructions to characterize the long-term influence of winter versus summer moisture on carbon-water relations in forested ecosystems.

Irrigation with saline waters is an agricultural practicethat is becoming increasingly common as competition for freshwater increases. In this thesis the mechanisms behind salinityand drought stress has been studied using data from fieldexperiments in combination with a modelling tool, theCoupModel. Measurements from field experiments on salinity,boron toxicity and drought stressed tomatoes grown during twoclimatically different seasons in the Arava desert, Israel,showed a linear relationship between relative growth andevapotranspiration, for all treatments and seasons. Data fromthe spring was used to concurrently simulate growth andtranspiration, hence accounting for feedback mechanisms betweenthe plant and the environment. Salinity stress was modelled asan osmotic effect (reduction of water uptake at high soilsalinities, W approach) or a toxicity effect (direct reductionof photosynthesis with soil salinity, G approach). Goodagreement between simulated growth and transpiration wasachieved with both salinity stress approaches, with twoexceptions. When growth and transpiration were simulated withthe W approach at different salinity levels, transpiration wasunderestimated at high stress. The G approach resulted in anunderestimation of growth at high water stress under moderatesalinity. A direct decrease of photosynthesis leads to adecreasing water-use efficiency with salinity while water-useefficiency remains constant with salinity when the salinitystress is modelled as a reduction in water uptake. Measurementsshowed decreasing water-use efficiency for the salinitygradient, explaining why the W approach was not applicable. Itwas not possible to detect any considerable differences betweenthree different approaches for water uptake tested in thestudy. <b>Keywords:</b>Water-use efficiency; osmotic effect; iontoxicity; semi-arid. / NR 20140805

The plant-soil nitrogen cycle plays a significant role in allocation of available N to plants, and improved understanding of N cycling helps sustainably increase fertilizer use efficiency. There are various processes (nitrogen mineralization and nitrification) involved in the availability and mobility of nitrogen in the soil. The primary objective of this study was to determine the NUE under contrasting nitrogen treatments over a period of five years. Additionally, we examined the effect of different N treatments on N mineralization and nitrification in conventional and organic farming systems. This project was funded by Agriculture and Food Research Initiative Competitive Grants Program Grant no. 2011-67019-30178 from the USDA National Institute of Food and Agriculture and by the Utah Agricultural Experiment Station. We established silage corn field plots in northern Utah, and silage corn was grown using ammonium fertilizers or manure composts over five years. Nitrogen use efficiency was found to be higher in ammonium sulfate fertilizer treatments as compared to compost treated soils. Nitrogen mineralization and nitrification rates were examined for soils from the silage corn field plots and also for additional soils from certified organic field plots receiving steer compost, steer manure and crop rotations. There was a significant overall nitrogen treatment effect for both conventional and organic rotational plots. Carbon mineralization rates were found to be higher in compost under conventional plots and manure under organic rotational plots as compared to control. There was no significant treatment effect found in gross mineralization and nitrification rates in 2015 and 2016. Gross nitrification rates were found to be the higher in AS200 treatment versus compost and control in 2016. Improved knowledge of the timing and rates of nitrogen supply is vital for improving NUE and for reducing excessive use of fertilizers while maintaining an acceptable yield. The optimization of fertilizer rates according to crop demand at different stages of growth will be helpful in the efficient management of available N especially for composts and manures.

Switchgrass (Panicum virgatum L.) has become an important bioenergy crop. Warm, winter temperatures in the southeastern USA allow for fall establishment and winter growth of cool-season legumes that may provide nitrogen to the spring perenniating crop of switchgrass. Data indicates variation due to year and location, but hairy vetch plots provided a greater nitrogen percentage in the subsequent biomass production of switchgrass. In 2011, switchgrass fertilized with 56 kg ha-1 N was greater than the control and in 2012 it was greater than the 28 kg ha-1 N treatment. Variation around the means prevented clear separation among other treatments. The data also showed that hairy vetch had the greatest volunteer frequency and cover percentage throughout the year. Data from the Dairy Farm showed no differences in yields due to a lack of field management the previous years and only ball clover increased its coverage over time.

Species in the genus Populus (poplars) have shown the potential to be utilized as short rotation woody crops for bioenergy production in the Southeast. A lack of knowledge on which poplar taxa perform best on marginal sites throughout the Southeast exists. Through measurement of relationships between growth metrics, water usage and physiological parameters of 2400 poplar trees, I was able to assess: 1) early rotation suitability of numerous poplar varietals to be grown as bioenergy feedstocks in northeast Mississippi, and 2) the effectiveness of early rotation physiological parameters in predicting future productivity and water usage. Overall findings from this study suggest that trees with D x M parentage may be best fit for large-scale plantation growth in the Southeast. They demonstrated low mortality and collectively grew the tallest of all taxa. Early-rotation physiology demonstrated mixed results in predicting future growth and water usage. Findings from this study will be used in future bioenergy feedstock selection.

No-till management for agronomic crop production is recognized as an effective practice to regain a portion of soil organic matter lost following decades of cultivation. Increasing soil organic matter sequesters C, conserves organic N and concomitantly improves soil quality. Objectives of this research were to: i) quantify C sequestration rate and N conservation with duration of continuous no-till; ii) measure C stratification with continuous no-till as an indicator of soil quality; and iii) evaluate the Illinois soil N test (ISNT) for its value to predict fertilizer N needs of corn in Virginia. Objectives i and ii were achieved by collecting soil samples from 63 production fields in the Virginia Coastal Plain that were managed using continuous no-till from 0 to 14 yrs. No-till management resulted in sequestration of 0.308 ± 0.280 Mg C ha⁻¹ y⁻¹ and conservation of 22.2 ± 21.2 kg N ha⁻¹ yr⁻¹ (0-15 cm). The C stratification ratio (0-2.5 cm: 7.5-15 cm) increased with increasing duration of continuous no-till (0.133 ± 0.056 yr⁻¹) due to the accumulation of organic matter at the soil surface indicating improved soil quality with continuous no-till management. Objective iii was addressed by conducting 29 on-farm fertilizer N response trials in major corn producing areas of Virginia with the duration of continuous no-till management ranging from 0 to 25 yrs. The ISNT values were significantly related to yield without fertilizer N (r² = 0.57; p<0.001) and relative yield (r² = 0.64; p<0.0001). We also found that the ISNT extracted a relatively consistent percentage of total soil N (16.3 ± 0.73 %) suggesting it is a poor indicator of labile N. Total soil N values did almost as well as the ISNT in predicting yield without fertilizer N (r² = 0.53; p = 0.0002), and equally well predicting relative yield (r² = 0.64; p<0.0001). Results do not suggest the ISNT is useful for measuring mineralizalbe N or improving fertilizer N recommendations in Virginia cropping systems. / Ph. D.

Flue-cured tobacco (Nicotiana tabacum) has been managed to optimize the yield of high-quality cured leaf while maintaining nicotine levels within a relatively narrow range based on the Regional Minimum Standards Program. Among the 3,000 plus alkaloids found in tobacco, nicotine accounts for greater than 90% of the total alkaloids produced in commercial tobacco varieties. Precious research has demonstrated an association with nicotine levels and cured leaf yield and quality. On March 16, 2018, the Food and Drug Administration issued a notice for proposed rulemaking to limit nicotine in combustible cigarettes to 0.3-0.5 mg nicotine per gram of tobacco (an approx. 98% reduction from current levels). Studies on achieving decreased levels of nicotine in flue-cured tobacco have been conducted since the mid-1900's and some success has been found through breeding and genetics. The FDA proposal suggested changes to standard agronomic production practices as a means of achieving their proposed nicotine levels. The primary objectives of this work are: (1) evaluate the impact of standard agronomic production practices on nicotine levels and the resulting yield and cured leaf quality and (2) quantify differences in nitrogen-use efficiency between conventional and low-nicotine flue-cured tobacco varieties. Results showed that the changes to production practices did not sufficiently lower nicotine levels to the range proposed by the FDA. The only publicly available low-nicotine variety, LA FC53, did not achieve the targeted nicotine level and the yield and cured leaf quality was severely reduced. The study of nitrogen-use efficiency quantified differences between conventional and low-nicotine flue-cured tobacco varieties. Proprietary, low-nicotine varieties developed from K 326 tended to have lower nicotine levels than LA FC53 with improved yield and cured leaf quality. Both yield and quality are associated with nitrogen-use efficiency and the nitrogen-use efficiency of the new low-nicotine lines was comparable to K 326 and better than the previously developed, low-nicotine standard variety. No evaluation of smoking characteristics of the cured tobacco from these studies was conducted and would be necessary for commercial utilization of low-nicotine flue-cured tobacco. / Master of Science / Flue-cured tobacco (Nicotiana tabacum) has been managed to optimize the yield of high-quality cured leaf while maintaining nicotine levels within a relatively narrow range based on the Regional Minimum Standards Program. Among the 3,000 plus alkaloids found in tobacco, nicotine accounts for greater than 90% of the total alkaloids produced in commercial tobacco varieties. Precious research has demonstrated an association with nicotine levels and cured leaf yield and quality. On March 16, 2018, the Food and Drug Administration issued a notice for proposed rulemaking to limit nicotine in combustible cigarettes to 0.3-0.5 mg nicotine per gram of tobacco (an approx. 98% reduction from current levels). Studies on achieving decreased levels of nicotine in flue-cured tobacco have been conducted since the mid-1900's and some success has been found through breeding and genetics. The FDA proposal suggested changes to standard agronomic production practices as a means of achieving their proposed nicotine levels. The primary objectives of this work are: (1) evaluate the impact of standard agronomic production practices on nicotine levels and the resulting yield and cured leaf quality and (2) quantify differences in nitrogen-use efficiency between conventional and low-nicotine flue-cured tobacco varieties. Results showed that the changes to production practices did not sufficiently lower nicotine levels to the range proposed by the FDA. The only publicly available low-nicotine variety, LA FC53, did not achieve the targeted nicotine level and the yield and cured leaf quality was severely reduced. The study of nitrogen-use efficiency quantified differences between conventional and low-nicotine flue-cured tobacco varieties. Proprietary, low-nicotine varieties developed from K 326 tended to have lower nicotine levels than LA FC53 with improved yield and cured leaf quality. Both yield and quality are associated with nitrogen-use efficiency and the nitrogen-use efficiency of the new low-nicotine lines was comparable to K 326 and better than the previously developed, low-nicotine standard variety. No evaluation of smoking characteristics of the cured tobacco from these studies was conducted and would be necessary for commercial utilization of low-nicotine flue-cured tobacco.

Development of winter wheat (Triticum aestivum L.) cultivars capable of more efficient uptake and utilization of applied nitrogen (N) has the potential to increase grower profitability and reduce negative environmental consequences associated with N lost from the plant-soil system. The first study sought to evaluate genotypic variation for N use efficiency (NUE) and identify lines consistently expressing high or low NUE under two or more N rates in a total of 51 N-environments. The results indicated that significant genotype by N rate interactions were frequently observed when trials utilized at least three N rates and identified wheat lines with high and stable yield potential that varied in performance under low N conditions. In addition, NUE was associated with above-ground biomass at physiological maturity were found to be both highly heritable across multiple N supplies. In the second study, two bi-parental mapping populations having a common low ('Yorktown') and two high (VA05W-151 and VA09W-52) NUE parents were characterized to dissect the genetics underlying N response. The populations were evaluated in eight N-environments and genotyped using single-nucleotide polymorphism data derived from a genotyping-by-sequencing protocol to identify quantitative trait loci (QTL) associated with high NUE. Six QTL for NUE were identified on chromosomes 1D, 2D, 4A, 6A, 7A, and 7D that were associated with N use efficiency. The QTL on 2D and 4A co-localized with known loci governing photoperiod sensitivity and resistance to Fusarium head blight (caused by the fungal pathogen Fusarium graminearum Schwabe), respectively. Three of the identified QTL (6A, 7A, and 7D) were associated with NUE in previous investigations, while the QTL on 1D was novel. The final experiment employed a small panel of soft red winter wheat lines to study the effects of photoperiod alleles on chromosome 1D (Ppd-D1) on yield-related traits under three or five N rates that were variably split over two growth stages in eight environments. The results validated the effect of a photoperiod sensitive allele (Ppd-D1b) that was associated with increased grain yield across N rates in half of the Virginia testing environments and under low N rates in all Ohio testing sites at the expense of grain N content. Yield advantages conferred by the Ppd-D1b allele were attributable to increased floret fertility and kernel number per spike. The findings from these studies have direct application for winter wheat breeding programs targeting NUE improvements. / Doctor of Philosophy / Wheat (Triticum aestivum L.) products account for a significant percentage of the total dietary calories and protein consumed globally. To meet production demands, wheat requires efficient nitrogen (N) management to ensure continued grower profitability and to reduce negative environmental impacts of N lost from agricultural systems. This dissertation sought to evaluate variation among wheat lines for N use efficiency (NUE), assess the performance of wheat lines under multiple N supplies, validate traits that are associated with NUE, investigate the role of photoperiod sensitivity genes on N response, and identify regions of the wheat genome associated with high N use efficiency. These studies were conducted using panels of winter wheat lines grown under two or more N conditions over a combined 32 location-years. Results of Chapter I identified variation in cultivar response to N rates was more frequently observed when a greater number of N rates were used in trials of wheat N response. The first chapter also identified variation among wheat lines for NUE and identified lines that consistently produce high grain yields over N-location-years. In addition, above-ground biomass at physiological maturity was found to be strongly associated with grain yield under all N rates and was highly heritable in both studies. Chapter II utilized a combination of genetic and observable trait data to perform genetic analysis in two bi-parental populations grown in eight Nlocation-years. The study identified reproducible and significant genetic markers associated with NUE for application in wheat breeding programs. Upon analysis of photoperiod sensitive versus insensitive wheat lines in Chapter III, photoperiod sensitive wheat lines had a significant yield advantage under N-limited conditions in Ohio and across N treatments in half of the Virginia testing location-years. This resulted from an increased number of kernels per spike and fertile florets in photoperiod sensitive wheat lines. Results from this dissertation suggest that active breeding and selection for N response may be achieved through the employment of high NUE genes and the continued identification of adapted high NUE wheat parental lines.