Purpose: The purposes of this research are to evaluate the effectiveness of a vaccine education module (VEM) in 1) contributing to new vaccine knowledge of university freshmen; and 2) identifying common vaccine concerns of university freshmen before and after completing the VEM. Methods: A VEM, including information about vaccine-preventable diseases, benefits of vaccines, principles of community or herd immunity, and where to go to get vaccinated, was created for freshman students. Student vaccine concerns were identified using a pretest/posttest quasi-experimental design using an online questionnaire before and after completing the VEM. After the VEM, participants were also asked to identify newly acquired knowledge. Results: Prior to viewing the VEM, the most common participant vaccine theme was the overall decrease of vaccination rates in the general population. After completing the VEM, the most common vaccine concern was the erosion of community immunity. The second most common theme were concerns about vaccine safety. Participants were also concerned about their own vaccination status. When asked what new knowledge they gleaned from the VEM, participants most frequently reported learning information about communicable diseases. Data were collected in the fall of 2019. Conclusions: Following the completion of the VEM, freshman university students reported fewer concerns about vaccine safety and more concerns regarding their current vaccination status. This demonstrates how a VEM contributes to vaccination knowledge and addresses vaccination concerns in university students.