This thesis describes the production of adenovirus-based vaccines containing codon-optimized genes from Nipah virus and Crimean-Congo Hemorrhagic Fever virus. Genes encoding envelope proteins from Crimean-Congo Hemorrhagic Fever Virus and Nipah Virus were codon-optimized for translation in human cells and constructed using a modified method of non-gapped gene synthesis, while the entire M segment encoding the glycoprotein precursor for Crimean-Congo Hemorrhagic Fever Virus was commercially synthesized. Genes were cloned into recombinant human adenovirus serotype 5 and the resulting viral particles were amplified, titred and analyzed for in vivo efficacy. Results show that a modified method of non-gapped gene synthesis is an effective and efficient method of producing antigen-encoded DNA and at a fraction of the cost and time required for commercial synthesis. Furthermore, adenovirus-based vaccines induce both cellular and humoral immune responses providing for a highly efficacious vaccine during potential disease outbreaks, where time to completion is of utmost importance. This study has shown that recombinant adenoviral vaccines for Crimean-Congo Hemorrhagic Fever virus and Nipah virus can be produced rapidly and efficiently from virtual DNA sequence to optimized recombinant vaccines in just eight months.

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Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1990. / Includes bibliographical references (leaves 444-454) / Microfiche. / xxi, 454 leaves, bound ill. 29 cm

Thesis (Ph. D.)--University of Hawaii at Manoa, 1991. / Includes bibliographical references (leaves 310-330) / Microfiche. / xiii, 330 leaves, bound ill., maps 29 cm

Thesis (M.A.)--University of Hawaii at Manoa, 2005. / Includes bibliographical references (leaves p. 213-227). / x, 227 leaves, bound ill. (some col.), maps (some col.) 29 cm

Research Doctorate - Doctor of Philosophy (PhD) / This thesis uses a transdisciplinary, sustainability-science approach to investigate the dialectics and potential for the Hunter Valley region of New South Wales, Australia, to make a transition to sustainability. The Hunter Valley, one of Australia’s historic food, wine and grain breadbaskets, is now home to over 50 opencut and underground black coal mines and is one of the world’s major coalmining and exporting regions. It is Australia’s largest black coal electricity generating region where six coal-fired power stations generate 40% of Australia’s electricity supply. The carbon intensity of the Hunter Valley’s economy makes the region Australia’s largest direct and indirect contributor to global carbon dioxide emissions. The region is a climate change hot spot that embodies the challenges and opportunities confronting Australia if it is to move towards a clean, renewable energy future and ecologically and socially-sustainable economy. The study examines the Hunter Valley as a complex adaptive socio-ecological system nested in an extended panarchy (Gunderson and Holling, 2002) that includes global energy systems and the ecosphere. The research examines the linked ecological and social health impacts of different scenarios for the Hunter Valley, comparing its current status – given the name Carbon Valley (Ray, 2005a) – with an alternative socio-ecological regime described by local residents as a Future Beyond Coal (CAN, 2006). This Future Beyond Coal is a regional manifestation of what Heinberg (2004) calls, at a global scale, a Post-carbon Society. Transdisciplinary sustainability-science is used to examine complex processes in which Hunter Valley residents are dealing with linked ecosystem-human health distress, while developing capacity for anticipating and forging change towards sustainability. They are also boosting the resilience of desirable states while challenging the perverse resilience of coal dependency. The thesis examines the potential for a ‘Just Transition’ to sustainability, a social and economic restructuring process which aspires to move the region’s socio-ecological relationships rapidly towards sustainability through protecting the wellbeing of vulnerable workers, communities and ecosystems. It investigates hegemonic relationships within coal communities, and the role popular education and social learning are playing in building a social movement for sustainability, a movement that links local, regional and global attractors and disturbances in order to change the basin of attraction from the current non-sustainable coal-dependent society to one that is ecologically sustainable and socially just.

Thesis (Ph. D. - Renewable Natural Resources)--University of Arizona, 1989. / Includes bibliographical references (leaves 57-62).

Thesis (M. S. - Geosciences)--University of Arizona, 1992. / Some pages are not numbered. Includes bibliographical references (leaves 36-37).

Thesis (M.A. - Anthropology)--University of Arizona. / Plates accompanied by guard sheets with descriptive letter press. Bibliography: leaves 191-196.