This pilot study was conducted to test if there are differences in occurrence of red-listed xylophagous fungi in forests dependent on degrees of human impact on the forests. Three main degrees of human impact was investigated: little impact (natural forest); medium impact (semi-natural forest) and high impact (monoculture plantation). These forests were compared in three geographically distinct areas within the same municipality, Vindeln. Within each forest type, three experimental plots were randomly selected, resulting in a total of 27 research plots. The study was conducted in northern Sweden, Västerbotten county in the following locations: Svartberget, Skatan and Kulbäcksliden. The pilot study found significant differences between natural forests and monoculture plantations. The natural forests harboured the most red-listed species, followed by the semi-natural forests, while the monoculture plantations had the fewest. The semi-natural was closer to the natural forest but did not yield significant differences when comparing the various areas. Despite the small dataset, the results are clear that the natural forests harbour the highest abundance of red-listed xylophagous fungi when comparing the degree of human impact in three different forest ecosystems.