11 |
Metabolomics in der Tumordiagnostik Massenspektrometrische Untersuchungen zum metabolischen Profil modifizierter Nucleoside /Bullinger, Dino, January 2008 (has links)
Tübingen, Univ., Diss., 2008.
|
12 |
Essays on the monetary transmission mechanismSuk, Jerry-Jeff January 2007 (has links)
Zugl.: Bern, Univ., Diss., 2007
|
13 |
Untersuchungen zur Vektorübertragung des Erregers der Apfeltriebsucht /Hoffmann, Andrea. January 2000 (has links)
Universiẗat Hohenheim, Diss.--Stuttgart, 2000.
|
14 |
Entwicklung eines ganzheitlichen Prognosemodells zur Kompensation von Varianzen in Prozessfolgen mittels Support Vektor MaschinenDecker, Markus, January 2007 (has links)
Zugl.: Stuttgart, Univ., Diss., 2007.
|
15 |
Essays on the monetary transmission mechanism /Suk, Jerry-Jeff. January 2008 (has links)
Zugl.: Bern, University, Diss., 2007.
|
16 |
Dynamic Factor Models in Macroeconomics with an Application to the Analysis of Technology ShocksSchetter, Ulrich. January 2007 (has links) (PDF)
Master-Arbeit Univ. St. Gallen, 2007.
|
17 |
The impact of macroeconomic factors on commodity pricesGao, Lin. January 2008 (has links) (PDF)
Master-Arbeit Univ. St. Gallen, 2008.
|
18 |
Herstellung und Anwendungen eines replikationsinkompetenten lentiviralen VektorsystemsJármy, Gergely. January 2001 (has links) (PDF)
Würzburg, Univ., Diss., 2001.
|
19 |
Integration von Kern-Methoden in das medizinische Monitoring /Lücke-Janssen, Daniela. January 2008 (has links)
Zugl.: Duisburg, Essen, Universiẗat, Diss., 2008.
|
20 |
Návrh a testování způsobů zvýšení účinnosti transfekce živočišných buněkŠtěpánková, Hana January 2018 (has links)
Master’s thesis deals with the design and testing of methods of increasing the transfection efficiency in mammalian cells. In this work a polyethylenimine (PEI) transfection method was used. The transfection efficiency was tested using MDA-MB-231 breast cancer cell line and pcDNA3.1-GFP-hMT-TOPO vector. We focused on optimization of PEI (1-6 µg) and plasmid DNA (1-3 µg) concentration, transfection time (0-48 h), number of cells per 9 cm2 (100,000-2 million) and medium composition. A complex containing linear penetration peptides (CPPs) KALA and GALA was assembled. Concentration of CPPs (7,5-45 µg) was optimized and a zeta potential and a size of the complexes were investigated. Due to the formation of large aggregates (over 1200 nm), modification with polyethyleneglycol (PEGylation) of PEI was performed. With the resulting complexes (~15 nm), transfection was performed. Transfection and apoptosis induction rates were compared between PEI and PEGylated PEI and as the best was chosen conjugate of PEGylated PEI (3 µg) with 45 µg of GALA and 3 µg pDNA with efficiency of 75%.
|
Page generated in 0.0679 seconds