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The host-parasite interaction during early filarial nematode migration is poorly understood. The objective of this study was to develop a model of early cutaneous filarid migration using Brugia pahangi in the jird (gerbil) host and measure the histologic and cytokine responses during this period. Male gerbils were intradermally inoculated in the left hindlimb with 100 B. pahangi L3 then necropsied at 3 hours, 24 hours, 3 days, 7 days, and 28 days post-infection. Larvae were recovered and tissues collected for histology and cytokine measurement. At 3 hours, most larvae (96.3%) were recovered from tissues associated with the infection site. Migration away from the infection site occurred within 24 hours. By 7 days, larvae were dispersed throughout the lymphatic system, including the spermatic cord lymphatics. Larvae were identified on histologic exam at all time points and were located in the dermis, muscle, lymphatic vessels, and lymph nodes. Predominantly neutrophilic inflammation was frequently present around larvae in the dermis and muscle at 3 and 24 hours. Levels of the cytokines IL-6, TNF, IFN-gamma, and IL-4 were measured in the spleen and popliteal and renal lymph nodes. IL-6 and TNF both showed a peak at 3 hours followed by consistent decline in all tissues. No clear increase in expression was appreciated for IFN-gamma. IL-4 remained low through 7 days and rose by 28 days in all tissues. These results indicate the ability of filarid L3 to rapidly migrate through host tissue and they support intradermal gerbil infection as a model for early filariasis. Cytokine analysis and histology indicated an acute host inflammatory response following initial infection, with Th2 polarization occurring later in the course of infection.

Summer pasture-associated obstructive pulmonary disease (SPAOPD), a naturally occurring airway disease of horses, is characterized by clinical exacerbation associated with exposure to pasture environment during the summer. Aeroallergens are believed to trigger exacerbation of SPAOPD, cytokines are likely associated with the anamnestic response to aeroallergens, and endothelin (ET)-1 is a potential mediator of airway obstruction. The goal of this dissertation was to describe and explore the interaction of aeroallergens triggering inflammation and T lymphocytes cytokine profile with the recruitment and activation of neutrophils and synthesis of ET-1 by mononuclear leukocytes and airway epithelial cells. The temporal pattern of clinical exacerbation was associated with hot and humid conditions and with increases in grass pollen and mold spore counts. Circulating concentrations of ET-1 were increased during clinical exacerbation of SPAOPD compared with remission and controls. Gene expression of ET-1 and cytokines, interleukin (IL)-8 and IL-4, but not interferon (IFN)-γ, tended to be greater in lungs of SPAOPD-affected than non-affected horses. The immunoreactive ET-1 distribution tended to be greater in airway tissues of affected horses. The putative aeroallergens, grass pollen & mold spores, and ET-1 induced neutrophil activation and chemotaxis in vitro. Putative aeroallergens induced IL-4 and IFN-γ expression and up-regulation of ET-1 release in mononuclear leukocytes. Cultures of airway epithelial cells were established under air-liquid interface and microgravity conditions and evaluated for differentiation. Cytokines IL-4 and tumor necrosis factor (TNF)-α induced up-regulation and directional (basolateral) release of ET-1 by differentiated airway epithelial cells, grown under air-liquid interface.

Thesis (M.S.)--West Virginia University, 2010. / Title from document title page. Document formatted into pages; contains vii, 56 p. : col. ill. Vita. Includes abstract. Includes bibliographical references (p. 35-36).

M.Comm. / The animal health industry in South Africa is static and products are of a generic nature. Most of the international players are represented in South Africa. The fact that there are so many players in the market leads to severe competition. In this study the possible application of relationship marketing in the animal health industry was investigated. Sanvet, one of the leading companies in the industry was used for the study. The company was established in September 1994 through an amalgamation of the animal health divisions of Agrihold and Premier Pharmaceuticals. In relationship marketing the focus moves from a short-term transactional approach to long-term relationship marketing. Relationship marketing is the integration of marketing, quality and customer service. The provision of quality customer service involves an understanding of what the customer buys and determines how additional value can be added to the product or service being offered. Quality is the link between what a customer expects and the customers perception of what is being offered. The four P's model is very limited and in relationship marketing three additional elements are being used. The elements are people, processes and the provision of customer service. Customer service creates a clearly differentiated and superior value proposition, and is the central focus of all the other elements. Through the acknowledgement of the contribution of people to getting and keeping customers, within the overall marketing mix, the company's competitive performance will be substantially enhanced. People can be categorised into the following groups based on their role in the company: contractors, modifiers, influencers and isolators. Although the human element is very important in customer service, no amount of energy from personnel can counter poor performance due to unsatisfactory processes. In relationship marketing not only the relationship with the target markets is being addressed but also the relationship with the intemal markets, referral markets, supplier markets, employee markets and influence markets. The employees of the company are the core of an internal marketing plan. Employees are seen as internal customers and jobs as internal products. The ideal form of marketing is to get customers to do the marketing on your behalf. Referral markets within the industry must be identified and marketing activities directed at them. The traditional adversarial relationship between suppliers and their customer must change to a new form of relationship based on co-operation. The competition between companies in their effort to attract suitably motivated and trained employees is increasing. Suitable employees are becoming very scarce resources. Companies must focus their marketing activities to ensure that they and the company are first choice for a potential employee. Financial markets, regulatory markets, the government and shareholders form the influence markets and marketing activities should be addressed to these markets to ensure a long-term relationship. This study was limited to investigating the relationship with the internal markets, referral markets and supplier markets. It was found that relationship marketing can be applied in the animal health industry. The recommendations in this study can be used to draw up a complete relationship marketing strategy for Sanvet.

Call number: LD2668 .T4 1957 A58 / Master of Science