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COMPLEMENTATION BETWEEN TEMPERATURE-SENSITIVE MUTANTS OF POLIOVIRUSWakeford, Laura, 1956- January 1987 (has links)
Conditional lethal mutants of poliovirus type 1 (Mahoney) were generated by treatment with the mutagen hydroxylamine. Temperature-sensitive mutants were selected by the replica plating technique at temperatures of 33°C (permissive) and 39°C (restrictive). New mutants were generated to achieve a larger population of mutants and also to generate additional RNA- mutants in this population. These mutants were characterized by two criteria: RNA synthesis and thermal stability. RNA synthesis is measured by the accumulation of labeled uridine incorporation into trichloroacetic acid (TCA) insoluble material. The thermal stability is determined by the difference in plaque forming units before and after treatment of the virion at 45°C. Complementation co-infections (5 MOI for each virus stock) were analyzed for the presence of the 150S virion particle of poliovirus after sedimentation through a linear sucrose gradient. Complementation is observed between RNA(+) mutants v.s. RNA(-) mutants, and between two RNA(-) mutants, but not between two RNA(+) mutants. Although reciprocal complementation has not been documented in this study some speculation on complementation is presented in this thesis.
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Gene expression and subgenomic RNAs of cucumber mosaic virusGordon, Karl H. J. January 1983 (has links) (PDF)
Includes bibliography (10 unnumbered leaves)
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Analysis of two highly expressed genes from Chlorella virus PBCV- 1 ; prote in characterization and the DNA sequences of the major capsid protein gene and the early/late 33-kDa protein geneGraves, Michael V. 06 December 1991 (has links)
Graduation date: 1992
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Expression of human herpesvirus 6 (HHV-6) genes in virus-infected cellsFok, Yuen-kei, Vivien., 霍沅琪. January 2005 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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THE EFFECTS OF A TEMPERATURE-SENSITIVE LIGASE ON MUTAGENESIS IN BACTERIOPHAGE-T4Wilson, Lois Bleicher, 1947- January 1974 (has links)
No description available.
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Molecular epidemiology of human coronavirus OC43 in Hong Kong /Lee, Paul, January 2007 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2007.
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Gene expression and subgenomic RNAs of cucumber mosaic virus /Gordon, Karl H. J. January 1983 (has links) (PDF)
Thesis (Ph. D.)--University of Adelaide, 1984. / Includes bibliographical references (10 unnumbered leaves ).
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Isolation and characterization of the Tipula Iridescent Virus capsid gene.Tajbakhsh, Shahragim, Carleton University. Dissertation. Biology. January 1988 (has links)
Thesis (Ph. D.)--Carleton University, 1988. / Also available in electronic format on the Internet.
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Expression of human herpesvirus 6 (HHV-6) genes in virus-infected cells /Fok, Yuen-kei, Vivien. January 2005 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2005.
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Cloning of alfalfa mosaic virusSternberg, Edward Allen. January 1982 (has links)
Thesis (M.S.)--University of Wisconsin--Madison, 1982. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 23-27).
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