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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Estudo QuÃmico do Fungo Antagonista Trichoderma harzianum / Chemical Study of the antagonistic fungus Trichoderma harzianum

NatÃlia Nogueira Saraiva 27 February 2009 (has links)
FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / A investigaÃÃo do potencial quÃmico de Trichoderma harzianum, um fungo antagonista, foi realizada. A composiÃÃo de Ãcidos graxos produzidos pelo fungo cultivado em Czapeck, peptona e caldo de batata, variando a fonte de carbono (glicose e manitol), nesse Ãltimo meio, foi determinada por CG/EM. Foram identificados os seguintes Ãcidos graxos na forma dos seus Ãsters metÃlicos: hexadecanÃico (C16:0), octadecanÃico (C18:0), 9-octadecenÃico (C18:1) e 9,12-octadienÃico (C18:2). Dos extratos orgÃnicos do fungo cultivado em peptona por 16 dias e BD (Batata-dextrose) por 24 dias foi possÃvel isolar o manitol e o composto antimicrobiano viridiofungina A, respectivamente. Extratos e fraÃÃes foram submetidos a testes antitumorais frente Ãs linhagens tumorais humanas MDA-MB435 (mama), HCT-8 (cÃlon) e SF-295 (glioblastoma) e cinco das fraÃÃes testadas apresentaram CI50 75% em pelo menos duas linhagens tumorais. A atividade alcooldesidrogenases (ADHs) de T. harzianum foi identificada e o microrganismo foi empregado como biocatalisador na reduÃÃo da (R)- carvona, acetofenona e seis alquilfenonas. Na maioria dos casos, os produtos de biorreduÃÃo foram obtidos. / The chemical potential of Trichoderma harzianum, an antagonist fungus, was investigated. The fatty acid composition of this fungus grown in Czapeck, peptone and potato, with different carbon source (glycose and manitol) in the later medium, was determined after GC/MS analysis. The following fatty acids, as their methyl esters, were identified: hexadecanoic (C16:0), octadecanoic (C18:0), 9-octadecenoic (C18:1) and 9,12-octadienoic (C18:2). From the organic extracts of the microorganism cultivated in peptone (16 days) and potatodextrose (21 days) broths, manitol and the antimicrobial viridiofungin A were isolated, respectively. Extracts and fractions were submitted to antitumor assays against the human cell tumor lines MDA-MB435 (mama), HCT-8 (colon) e SF-295 (glioblastoma) and five of them showed IC50 75 % in two or three cell lines. Alcohol dehydrogenases (ADHs) activity of T. harzianum was identified and this microorganism was used as biocatalyst in the reduction of (R)-carvone, acetophenone and six akylphenones. In most cases, the products of the bioreductions were obtained.

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