Field and greenhouse experiments were conducted to determine the effect of auxin injury on soybean and cotton due to spray hose material, formulation and cleanout procedures on auxin equipment cleanout. Visual estimations of injury (VEOI) in wheat, height reduction, and yield reduction due to rimsulfuron and glyphosate titration was higher when compared to rimsulfuron only treatments with respect to 1/2X through the 1/256X treatments. Sequestration of 2,4-D within agricultural hose types did differ due to hose type and is confirmed by analytical testing, but field observation of wheat did not show differences among treatments due to VEOI, height reduction or yield reduction. Using soybean as a bio-indicator, differences did occur with respect to dicamba sequestration in agricultural hose types with respect to VEOI, height reduction, node reduction, yield reduction and ppm analyte retained. Results indicate chemical makeup of hose type in determination of ppm analyte dicamba retained. Cleaning procedures of water or ammonia do not prove to be different with respect to VEOI, height reduction, yield reduction or ppm analyte retained. Sequestration of 2,4-D within valved manifold systems and using water or ammonia as cleanout procedures in conjunction with rinse procedures did not show differences with respect to VEOI, height reduction, nodes above cracked boll (NACB), yield reduction or ppm analyte retained. It was not until standard 2,4-D applications were applied in field experiments when differences were observed. Deactivation of dicamba and 2,4-D using the Fenton procedure within various rates, showed an interaction with respect to VEOI, height reduction, node reduction, yield reduction and ppm analyte. Using soybean as a bio-indicator showed differences with the Fenton procedure deactivating the dicamba analyte in the 1/16X, 1/64X and 1/256X rate with respect to VEOI, height reduction, node reduction, yield reduction and ppm analyte retained. Using cotton as a bio-indicator showed differences with the Fenton procedure deactivating the 2,4-D analyte in every rate with respect to VEOI, height reduction, yield reduction and ppm analyte.