Scenedesmus obliquus is a freshwater microalga which has high lipid content and biomass productivity. It is regarded as a promising species for production of biodiesel and other valuable organic compounds. Given the high cost of using potable water and commercial fertilizers, the use of municipal wastewater as algal growth medium is attractive in view of its constituent organic carbon and inorganic nutrients, including nitrogen and phosphorus. Investigating the mixotrophic cultivation of S. obliquus in an imitation municipal wastewater, the results of this study showed that: (1) The unmodified imitation wastewater by itself as expected yielded poor S. obliquus growth owing to its pH significantly decreasing to 3.5 as caused by the presence of Ammonium Chloride in the wastewater, inhibiting cell growth; (2) Adding either Acetic Acid or Sodium Acetate to the wastewater medium maintained its pH at 6.5 to 7.0, and its algae biomass on day 6 increased significantly by 212% and 194%, respectively; (3) Adding either Acetic Acid or Sodium Acetate to the wastewater medium maintained its pH at 6.5 to 7.0, and its algae biomass during exponential phase (day 4) significantly exceeded that in the MF control by 220.6% and 165.8%, respectively, while its algae biomass during saturation (day 6) significantly exceeded that in the MF control by 60.8% and 51.5%, respectively; and (4) Addition of NaNO₃ to the wastewater to match the level of N in the MF medium improved the algae biomass by 10%. This study developed ways for how the successful mixotrophic cultivation of S. obliquus in municipal wastewater could be achieved.