Global ETD Search

1	Sérologická diagnostika borelióz / Serological diagnosis of borreliosis deseases Sližová, Ivana January 2016 (has links) The aim of present master’s thesis was to compare the results of serological methods for diagnosing borreliosis that are commonly used in Spadia laboratories (ELISA, immunoblots) in terms of recommendation on how and when to indicate and interpret them. The theoretical part is focusing on the characteristics and history of borreliosis, microbiological description of Borrelia, immune system and pathogenesis of the disease as well as the therapy and prevention. The experimental part is focusing on the analysis of results obtained from common examinations of antibodies to Borrelia made in Spadia Lab laboratories from January 1st 2014 to December 31st 2015. Screening of antibodies to Borrelia made by ELISA in IgM and IgG was done for all samples according to recommendation of CDC. In 2014 the ELISA screening was done using ELISA kits from Euroimmun and Evolis sample processors whereas in 2015 it was done using DiaSorin’s CLIA kits on Liaison analyser. Positive results were then confirmed by Westernblot or lineblot alternatively if the physician did not ask otherwise. It must be remembered that ELISA and Westernblot belong among serological methods that are using antibodies, i.e. substances produced by the immune system. The immune system plays the key role in protecting the body against infection and the antibodies are its important tool. Serological methods belong among immunoassay methods, which is still not standardized. Diagnosis of infections cann‘t be based only on antibody testing. It is necessary to assess the results in the context of the entire clinical picture, history and in the case of antibodies it is recommended retesting with an interval.
2	Development of enzyme linked immunosorbent assay for Tripeptidyl-peptidase II and comparison with commercial kits Tilda, Jonnergård January 2024 (has links) Tripeptidyl peptidse II (TPPII) is an enzyme forming a complex of 4,5 MDa. It is located in the cytosol and participates in protein turnover where it degrades oligopeptides to smaller peptides and tripeptides. Moreover, some of its products might take part in antigen presentation through MHC-class 1. High expression of the enzyme is believed to correlate with tumor progression. A decreased concentration seems to contribute to increased susceptibility to infection. The aim of this project was to develop an enzyme linked immusorbent assay (ELISA) specific for TPPII and compare it to two commercial kits. TPPII was detected through western blot and activity assay to ensure which samples were containing the enzyme. Samples with purified enzyme from different species, erythrocyte lysate and plasma were used to develop an indirect ELISA as well as compare all the assays and evaluate which one performed the best. The commercial kits were based on sandwich and competitive techniques. It was observed that the developed ELISA was able to detect the purified enzyme of different concentrations whereas the commercial kits could not. On the other hand, the commercial kits were able to generate their own standard curve which leads to suspicion that these might be non-specific to TPPII. Though, all assays did detect the enzyme in erythrocyte lysate. This study presents that it is possible to develop an ELISA specific for TPPII using an indirect technique which also performed better than commercial kits. ELISA TPPII westernblot activity assay antibody Biomedical Laboratory Science/Technology
3	Proteomische Analyse eines in-vitro-Modells für die interstitielle renale Fibrose: Der osmotische Stress als Fibrose-triggernder Faktor / Proteomic analysis of in vitro Model for interstitial renal fibrosis: The osmotic stress as fibrosis triggering factor Lahrichi, Loubna 07 August 2012 (has links) No description available. 610 Medizin, Gesundheit MED 418 Medicine Renale Fibrose Nierenfibroblasten 2D-Gelektrophorese Massenspektrometrie Westernblot Immunfluoreszenzfärbung ER-Stress-Proteine UPR-Pathway Fibrosemarker oxidativer Stress osmotischer Stress Renal fibrosis kidney fibroblasts 2D electrophoresis mass spectrometry Western blot immunofluorescence staining ER stress proteins UPR pathway fibrosis marker oxidative stress osmotic stress 44.88

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