Objective: To study the accuracy and value of immediate wet preparation (WP) procedure on effusion and washing cytologic specimens. Study Design: Two hundred specimens were identified over 3 months in our cytology laboratory, including 102 pleural effusion, 59 peritoneal effusion, 28 pelvic washing and 11 pericardial fluid specimens. WP slides were prepared, stained with toluidine blue (TB) and evaluated. Findings were reported as negative, suspicious or positive for malignant cells. For negative specimens, the remaining prepared slides were stained together. For suspicious or positive interpretation, slides were stained separately. Accuracy and additional benefits from this immediate triage step were studied. Results: Interpretation of slides resulted in 152 negative, 34 positive and 14 suspicious for malignancy. Analysis for additional values resulted in immediate interpretation relayed to clinicians, additional fluid centrifuged for adequate sediment in samples with scant cellularity, selection of bloody specimens for acid washing procedures, selection of cases to optimize cell block preparation when pivotal histologic evaluation or immunohistochemistry was anticipated and selection of cases for potentially needed ancillary studies. Accuracy, sensitivity, specificity and positive and negative predictive values were high. Conclusion: WP using the TB is accurate, sensitive and highly specific and has considerable value beyond segregating potential neoplastic cases.

The diploma thesis deals with electrode materials for lithium-ions accumulators, concretely layered materials prepared via new methods. The main objective of this work is dealing with new procedures prepare electrodes of newly developed layered electrode materials and subsequently their measure. Another challenge was a theoretical analysis of newly developed layered electrode materials for positive and negative electrodes and their preparation of new procedures. In this work the detailed procedures for the preparation of individual electrodes, electrolytes and other issues associated with these preparations. There were describing batteries of general, primary and secondary lithium cells, fuel cells, Lithium-ion batteries, layered materials forming the electrodes and of course the history these cells. Practical work is focused on separate measurements layered electrode materials prepared by new processes and assessment of results for individual layered materials. In the practical part has been made that the newly prepared layered electrode materials offer higher capacity and voltage.