Spelling suggestions: "subject:"wholecell immunohistochemical method"" "subject:"wholecell immunocytochemistry method""
1 |
Seasonal dynamics of unicellular diazotrophs in the upstream Kuroshio and the northern South China SeaYong, Tze-Ching 05 March 2011 (has links)
Seasonal dynamics of unicellular diazotrophs were investigated in the upstream Kuroshio and the northern South China Sea (SCS). Unicellular diazotrophs had been postulated as an important N2-fixing contributor for the phenomenon of N* in the SCS where abundances of filamentous Trichodesmium and Richelia were scarced. Samples were collected during four cruises between August 2008 and August 2009 in summer (CR1310 and CR910), winter (CR886), and late spring (CR899), respectively. Sampling stations located between 21¢XN-22¢XN and 116¢XE-122¢XE in the upstream Kuroshio off southeast Taiwan and covering the shelf and basin waters of the northern SCS. The abundance of the unicellular diazotrophs was determined using whole-cell immunocytochemical method in which antibody of nitrogenase was used as the probe. Cells containing nitrogenase can be visualized and counted after the antigen-antibody reaction under microscope. Unicellular diazotrophs were classified to four types according to their sizes and shapes. For diameters of those with 1-3 £gm and in coccoid shape are called 1-3 £gm C, diameters of 1-3 £gm and in rod shape are called 1-3 £gm R, diameters of >3-10 £gm and in coccoid shape are called >3 £gm C, and diameters of >3-10 £gm and in rod shape are called >3 £gm R.
Surface abundance of the unicellular diazotrophs was highest in winter in both the Kuroshio and the SCS, followed by summer, and was least in late spring. Among four cell types, 1-3 £gm C usually was the most abundant group, followed by 1-3 £gm R and >3 £gm R, and was least for the group of >3 £gm C. The abundances between groups of 1-3 £gm C and 1-3 £gm R were positively correlated. Likewise, the abundances between >3 £gm C and >3 £gm R were positively correlated. However, the total abundance of small cells (1-3 £gm C+R) was not significantly related to the large cells (>3 £gm C+R). During summer and late spring, the abundance of unicellular diazotrophs in the SCS was 1.3-2 times of that in the Kuroshio. However, in winter the abundance in the Kuroshio was 1.2 times of that in the SCS. Surface water temperature was found negatively correlated to the abundance of 1-3 £gm C, >3 £gm C, >3 £gm R, and large cells (>3 £gm C+R), respectively. Significant correlations among surface water temperature and surface chlorophyll a, [NO2+NO3], SRP and N:P ratio implicated that the dynamics of cell abundances could be attributed to the correlated ecological variables of surface water temperature. The dynamics for the abundances of >3 £gm C, >3 £gm R, and large cells (>3 £gm C+R) were suggested to relate with the fluctuation of SRP concentration. Unicellular diazotrophs accounted for 60-90 % of total unicellular cells in terms of cell number. Vertical distributions of unicellular diazotrophs in the Kuroshio and the SCS were in similar trends, with maximum abundance in deep water during summer and late spring, and on surface water during winter.
|
Page generated in 0.1565 seconds