Introduction: Isokinetic testing is used in rehabilitation settings on a regular basis, yet there is a lack of consistency in rest period usage among protocols. Purpose: The purpose of this study was to establish an optimal rest period that would allow reproducibility of strength during a common isokinetic strength-test. Methods: Twentyseven healthy college-aged males underwent isokinetic strength testing to determine peak torque at 60, 180 and 300 deg/sec, respectively. Work:rest ratios of 1:3, 1:8 and 1:12 were counterbalanced between sets. A 3 X 3 repeated measures ANOVA was used to analyze the data. The p < .05 level of significance was used for all tests. Results: There was no significant difference in either knee extension or knee flexion peak torque when comparing work:rest ratios. Conclusion: These findings suggest that a 1:3 work:rest ratio is sufficient during a common isokinetic strength test.