Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-10-10T16:40:48Z No. of bitstreams: 1 2011 - Raquel Rodrigues Costa Mello.pdf: 1289647 bytes, checksum: f0d06ec4ba47cab82e19761e6bf670ec (MD5) / Made available in DSpace on 2016-10-10T16:40:48Z (GMT). No. of bitstreams: 1 2011 - Raquel Rodrigues Costa Mello.pdf: 1289647 bytes, checksum: f0d06ec4ba47cab82e19761e6bf670ec (MD5) Previous issue date: 2011-08-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The Sindhi breed has excellent adaptability to adverse weather conditions and management, and good ability to milk production. Although many researches will be developed with Sindhi breed animals, such experiments are focused mainly on productive aspects, and researches involving the reproductive tract are still scarce in the Sindhi Zebu breed. Thus, the aim of this study was to evaluate the reproductive parameters of Sindhi cows, aiming to improve the efficiency of FTAI protocols in this breed. To this end, sixteen Sindhi cows with body condition score between 3,0 and 4,0 (scale from 1 to 5) were divided into two evaluation groups: In group I (BE protocol, n=8) animals received on day 0 an intravaginal P4 device and an application of 2mg of estradiol benzoate. On day 8 the device was removed and applied 10mg of Dinoprost (PGF2?), and on day 9, applied 1 mg of estradiol benzoate, and were fixed-time artificial inseminated 36 h later; in group II (BE + eCG protocol, n=8) animals were submitted through same treatment, and on day 8 were applied 400 IU of eCG. From day 0 for both treatments, the animals were examined daily by trans-rectal ultrasound device equipped with a 7,5MHz linear transducer (CHISON? D600 VET). This evaluation was performed up to ovulation. At each assessment, the largest follicles of each day (?4 mm) were measured. It used analysis of variance (ANOVA) to evaluate the results, and the means determined by Tukey test, with significance level of 5%. For data analysis, it was observed that there was no statistical difference (P>0.05) for groups I (Treatment I: no eCG) and II (Treatment II: 400 IU eCG) concerning to emergence of follicular wave, 4.5 ? 1.4 and 3.8 ? 0.3 days; interval between the emergence of the wave and ovulation, 5.8 ? 1.4 and 7.1 ? 0.3 days; interval between the emergence of the wave and implant removal, 3,5 ? 1,4 and 4,1 ? 0,3; interval between implant removal and ovulation, 74.0 ? 11.8 and 82.5 ? 4.2 hours; diameter of the dominant follicle (DF) at the time of implant removal, 7.5 ? 2.5 and 6.6 ? 1.5 mm; diameter maximum of DF, 10.47 ? 8.85 and 3.7 ? 1.2 mm; growth rate of the DF, 0.90 ? 0.83 and 0.7 ? 0.6 mm/day and ovulation rate, 75 % (6/8) and 100% (8/8), respectively. Regarding to pregnancy rate, it was observed that there was statistical difference (P<0.05) between protocols I and II (0% and 50%, respectively). Therefore, we conclude that the use of eCG in Sindhi breed cows was not effective in improving the reproductive parameters examined, perhaps because the cows are cycling at the beginning of treatment and had good body condition score during the experiment. On the other hand, there was an increase in pregnancy rate with the use of eCG, indicating that this gonadotropin may be a useful tool in FTAI protocols in this breed. / A ra?a Sindi apresenta excelente adaptabilidade ?s condi??es adversas de manejo e clima, e boa capacidade de produ??o de leite. Embora muitas pesquisas venham sendo desenvolvidas com animais desta ra?a, tais experimentos se concentram predominantemente em aspectos produtivos, pesquisas envolvendo a ?rea reprodutiva ainda s?o escassas na ra?a Sindi. Portanto, o objetivo deste trabalho foi avaliar os par?metros reprodutivos de vacas Sindi, buscando melhorar a efici?ncia dos protocolos de IATF nesta ra?a. Para tanto, 16 vacas da ra?a Sindi, com escore de condi??o corporal entre 3,0 e 4,0 (escala de 1,0 a 5,0) foram divididas em dois grupos de avalia??o: no grupo I (esquema BE, n=8), os animais receberam no dia 0 um implante intravaginal impregnado com 1,9g de progest?geno e uma aplica??o de 2mg de benzoato de estradiol. No dia 8 o implante vaginal foi removido, sendo aplicados 10mg de Dinoprost (PGF2?) e, no dia 9, aplicado 1mg de benzoato de estradiol, sendo realizada a IATF 36 horas ap?s; no grupo II (esquema BE + eCG, n=8), os animais passaram pelo mesmo tratamento, sendo que no dia 8 foram aplicadas 400UI de gonadotrofina cori?nica eq?ina (eCG). A partir do dia 0, para os dois tratamentos, os animais foram examinados diariamente por via trans-retal com aparelho de ultrassom (CHISON? D600 VET) equipado com um transdutor linear de 7,5 MHz. Esta avalia??o foi realizada at? o momento da ovula??o. Em cada avalia??o, os maiores fol?culos de cada dia (?4 mm) foram mensurados. Foi utilizada a an?lise de vari?ncia (ANOVA) para avalia??o dos resultados, sendo as m?dias determinadas pelo teste de Tukey, com n?vel de signific?ncia de 5%. Pela an?lise dos dados, observou-se que n?o houve diferen?a estat?stica (P>0,05) para os grupos I e II na emerg?ncia da onda folicular, 4,5 ? 1,4 e 3,8 ? 0,3 dias; no intervalo entre a emerg?ncia da onda e a ovula??o, 5,8 ? 1,4 e 7,1 ? 0,3 dias; no intervalo entre a emerg?ncia da onda e a retirada do implante, 3,5 ? 1,4 e 4,1 ? 0,3; no intervalo entre a retirada do implante e a ovula??o, 74,0 ? 11,8 e 82,5 ? 4,2 horas; no di?metro do fol?culo dominante (FD) na retirada do implante, 7,5 ? 2,5 e 6,6 ? 1,5 mm; no di?metro m?ximo do FD, 10,47 ? 3,7 e 8,85 ? 1,2 mm; na taxa de crescimento do FD, 0,90 ? 0,7 e 0,83 ? 0,6 mm/dia e na taxa de ovula??o, 75% (6/8) e 100% (8/8), respectivamente. Com rela??o ? taxa de prenhez, foi observada diferen?a estat?stica (P<0,05) entre os grupos I e II, sendo 0% (0/8) e 50% (4/8), respectivamente. Portanto, conclui-se que o uso do eCG em f?meas bovinas da ra?a Sindi n?o foi efetivo em melhorar os par?metros reprodutivos analisados, talvez pelo fato das vacas apresentarem bom escore de condi??o corporal durante a realiza??o do experimento. Por outro lado, houve um aumento na taxa de prenhez com o uso do eCG, indicando que este pode ser uma ferramenta ?til em protocolos de IATF nesta ra?a.