Zero-exchange system doesn¡¦t discharge the nutrient-rich pool water to the environment, so it doesn¡¦t cause the pollution. Besides, nutrients in the water can be re-used and the amount of water used can be reduced. In shrimp pond, ammonia and nitrite will accumulate during the period of cultivation because of feeding large amount of protein-rich feed. In traditional culture system, a great deal of water was used to solve the problem. In zero-exchange system, nitro-bacteria were used to convert ammonia and nitrite into nitrate, the need of water exchange was much-reduced. In this study, shrimps (Litopenaeus vannamei) were cultured in FRP tanks, and organic particles were suspended by strong aeration. There are four experiments (1) substrate: net-liners, net-pockets, mats, coral granules and blank control. (2) density: 100, 200, 300 and 400 /m2. (3) feeds: White shrimp feed (contain 39% protein), tiger shrimp feed (contain 39% protein) and Kuruma prawn feed (contain protein 54%). (4) zero-exchange water and change water (35% ponds of water / week). Net-pockets have the highest yield and final weigh and lowest FCR, and the net-liners isolate highest amount of the suspending particle. Shrimp final weight reduces as the stocking density increases, white shrimp grows slower when density excess 200 /m2. Feed contains higher protein can improve the efficiency of feed used by shrimp, but the cost of the feed become higher. Use the tiger shrimp feed (containing 39% protein) only can also reach high yield. Except orthophosphate, ammonia, nitrite and nitrate are not different from the exchange system. When compared with those of the zero-exchange system survival, final weigh and FCR are not different between two the treatments, but yield is higher in zero-exchange system. In conclusion, shrimp cultured in zero-exchange system with density of 200 /m2, substrate of net-pockets and feeds the contain 39% protein can reveal optimum yield.