The experiment was based on the feeding of feed additive, which was composed of vitamins, methionine, zinc and copper in organic form (amino acid complex n-hydrate). The experiment was 14 horses divided into two groups (test and control group). The experimental group was the basic ration, which also received a control group, added over a period of nine months, a feed additive. During the experiment, 5 samples were taken from which the detected concentration of some mineral elements (Ca, Fe, Mg, Cu, Zn) and various biochemical parameters (glucose, aspartate aminotransferase, urea, bilirubin, alkaline phosphatase). Administration of a feed additive was a statistically significant (P <0.05) increase in the zinc content of the original 5828 mg +- 1.67 10.57 +- 0.79 four milligrams. The copper content in the control sampling was 10.737 +- 2.46 and statistically significantly increased to 12.698 +- 1.74 mg. Check sampling contained 4.58 +- 0.56 mmol / l glucose, the last collection and showed no significant difference statistically insignificant (P <0.01) was 4.45 +- 0.46 mmol/l. In the control sampling was determined the average number of aspartate aminotransferase to 2.46 +- 0.48 mikromol/l, which is due to the administration of feed additives statistically insignificant (P <0.01) increased to 3.37 +- 0.71 mikromol/l.. Blood urea is statistically insignificant (P <0.01) did not change much, the initial value of 5.86 +- 1.20 mmol / l decreased to 5.75 +- 1.17 mmol / l. Bilirubin is statistically insignificant (P <0.01) without significant deviation from the initial 14.61 +- 5.45 mikromol/l slightly increased to 14.78 +- 6.41 mikromol/l.. Alkaline phosphatase statistically significant (P <0.05) increased from 1.98 +- 0.34 mikromol/l to 3.18 +- 0.66 mikromol/l.