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Transplantation of Human Embryonic Stem Cells to a damaged Human Cornea an in vitro study

Corneal dystrophies are commonly referred to as an congenital condition. Surgical complications are usually worse then the primary dysfunction and patients need to go through large surgical process. Only 25 % of the patients reach driving licence vision after a surgery. The aim of this study was to investigate if the application of human embryonic stem cells (hESc) could replace epithelial cells of the human cornea. Corneal markers such as cytokeratins CK3, CK15, CK19 and Pax - 6 were analysed by immunohistochemistry. HES - Cellect was used as indication of  stem cell potential of the transplanted cells. hESc transplanted onto the cornea could be seen to attach and expand dominantly  towards Bowman’s membrane. Human embryonic stem cells in culture were relatively positive for markers, contradictionally stem cells  in the epithelial trails lost their stem differentiation  potential  and appeared to be negative for all markers used in these trails. Optimization of stem cells differentiation into epithelial which may in the future may gives us the ability to perform clinical applications with successful outcome.

Identiferoai:union.ndltd.org:UPSALLA1/oai:DiVA.org:sh-3672
Date January 2010
CreatorsGurajada, Deepthi
PublisherSödertörns högskola, Institutionen för livsvetenskaper
Source SetsDiVA Archive at Upsalla University
LanguageEnglish
Detected LanguageEnglish
TypeStudent thesis, info:eu-repo/semantics/bachelorThesis, text
Formatapplication/pdf
Rightsinfo:eu-repo/semantics/openAccess

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