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Previous issue date: 2015-03-11 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O objetivo deste estudo foi realizar a extra??o ?leo de r?-touro ?leo de r?-touro e desenvolver uma emuls?o adequada para uso t?pico do mesmo. Duas amostras de ?leo fora obtidas por diferentes m?todos, sendo uma por extra??o a quente e outra utilizando solvente org?nico. As amostras foram fisioquimicamente caracterizadas por t?cnicas de titula??o e seus compostos identificados atrav?s de cromatografia gasosa acoplada a espectrometria de massas (GC/EM). O equil?brio hidrif?lico-lipof?lico requerido (EHLr) do ?leo de r?-touro foi determinado e em seguida um diagrama de fases constru?do. A estabilidade da emuls?o de uso t?pico contendo diferentes adjuvantes farmac?uticos foi determinada. A an?lise de citotoxicidade do ?leo de r?-touro in natura e na emuls?o de uso t?pico foi realizada atrav?s do ensaio de MTT, utilizando linhagem de fibroblastos normais (3T3) e de melanoma (B16F10). O rendimento da extra??o a quente foi de 60,6%. Os principais compostos insaturados encontrados foram o ?cido eicosapentaen?ico (17,6%) e ?cido araquid?nico (8,4%). O estudo de EHLr demonstrou a presen?a de sistemas est?veis com EHL entre 12 e 13,5 e o diagrama de fases revelou a predomin?ncia de sistemas caracterizados como emuls?o (62%). A emuls?o t?pica apresentou tamanho de got?cula igual a 390 nm, polidispers?o de 0,05, potencial zeta -25 mV e manteve-se est?vel durante os 90 dias avaliados. O ?leo de r?-touro e a emuls?o t?pica n?o apresentaram citotoxicidade frente ? linhagem de c?lulas 3T3. No entanto, estes sistemas inviabilizaram significativamente (p > 0,05) o crescimento das c?lulas B16F10. Em conclus?o, o ?leo de r?-touro apresenta caracter?sticas qu?micas desej?veis para o desenvolvimento de sistemas terap?uticos de uso farmac?utico e/ou cosm?tico. / The skin is one of the largest organs of the human body and accounts for about 16% of body weight. The body protection against the external environment microorganisms is one of its most important functions, however is necessary that the skin remain intact for this function be exercised, so that when there is an injury on the skin, the process of restructuring needs to be starts, however this restructuration may also be compromised due to some diseases, justifying even more the need for the development of topical products that promote or accelerate the skin healing. Thus the aim of this study was to extract bullfrog oil and to develop a suitable topical emulsion. Two different oil samples were extracted by hot or organic solvent process. Titration techniques and gas chromatography- mass spectrometry were used to characterize the bullfrog oil. The required hydrophile-lipophile balance (HLBr) of bullfrog oil was determined and a pseudo-ternary phase diagram was constructed. The stability of the topical emulsion was evaluated. Then, cellular viability was determined by MTT assay using normal fibroblasts (3T3) and melanoma (B16F10) cells lines. The hot extraction yield was 60.6%. The major polyunsaturated compounds found were Eicosapentaenoic acid (17.6%) and Arachidonic acid (8.4%). HLBr study demonstrated the presence of stable systems with HLB ranging from 12.1 to 13.5 and the pseudo-ternary phase diagram showed mainly emulsion systems (62%). Topical emulsion showed 390 nm, polydispersity 0.05, zeta potential -25 mV and remained stable for ninety days. The bullfrog oil and topical emulsion did not showed citotoxicity in normal fibroblasts cells. However, these systems showed significantly inhibition of melanoma cells growth. In conclusion, the bullfrog oil presented desirable chemical characteristics required to be used for the development of a pharmaceutical and cosmetic products.
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/20611 |
| Date | 11 March 2015 |
| Creators | Machado, Lucas Amaral |
| Contributors | 38878240400, http://lattes.cnpq.br/6907806915889763, Reynaud, Franceline, 00344484971, http://lattes.cnpq.br/2561590161798118, Giordani, Raquel Brandt, 00104164042, http://lattes.cnpq.br/5032750980466610, Silva J?nior, Arn?bio Antonio da, Egito, Eryvaldo S?crates Tabosa do |
| Publisher | Universidade Federal do Rio Grande do Norte, PROGRAMA DE P?S-GRADUA??O EM CI?NCIAS FARMACEUTICAS, UFRN, Brasil |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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