The expression of the adenovirus (Ad) genome is co-ordinately regulated and the various early regions of the genome are known to exert feedback controls on each other. The region E1B is known to encode functions which are required for oncogenic transformation by adenovirus yet its potential regulatory role is poorly understood. In this study the regulatory role(s) of the region E1B was investigated at the level of RNA accumulation. The Northern blot technique using various cloned early regions as probes and RNAs from E1B mutants Ad 12, Ad 2 and Ad 5 were used. The levels of region E1A specific RNAs were found to be aberrant. Thus for Ad 12 cyt 68 and Ad 2 dl 250 the levels were higher than wild type ones at late times whereas for Ad 5 dl 313 hr-6 the levels were consistently lower than Ad 5. This implies that the region E1B normally encodes functions which are involved either directly or indirectly in the efficient accumulation of region E1A specific RNA. Other regions also seemed to be affected in these mutants. In cyt 68, region E1B RNA was higher than wild ones at late time, indicating an auto-regulatory mode of control for this region. The expression of region E3, E4 and LS RNA were all perturbed by the E1B mutation as some of the mutants either accumulated higher or lower than wild type levels of RNA. / Thesis / Master of Science (MS)
| Identifer | oai:union.ndltd.org:mcmaster.ca/oai:macsphere.mcmaster.ca:11375/23068 |
| Date | 09 1900 |
| Creators | Caussy, Deoraj |
| Contributors | Mak, Stanley, Biology |
| Source Sets | McMaster University |
| Language | English |
| Detected Language | English |
| Type | Thesis |
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