Animal behaviours are demonstrably governed by sensory stimulation, previous
experience and internal states like hunger. With increasing hunger, priorities shift towards
foraging and feeding. During foraging, flies are known to employ efficient path integration
strategies. However, general long-term activity patterns for both hungry and satiated flies
in conditions of foraging remain to be better understood. Similarly, little is known about
how permanent contact chemosensory stimulation affects locomotion. To address
these questions, we have developed a novel, simplistic fly activity tracking setup—
the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of
walking behaviour, of several flies in parallel, with all arena surfaces covered by a
uniform substrate layer. We tested two constellations of providing food: (i) in single
patches and (ii) omnipresent within the substrate layer. Fly tracking is done with FIJI,
further assessment, analysis and presentation is done with a custom-built MATLAB
analysis framework. We find that starvation history leads to a long-lasting reduction
in locomotion, as well as a delayed place preference for food patches which seems to
be not driven by immediate hunger motivation.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84329 |
| Date | 27 March 2023 |
| Creators | Mahishi, Deepthi, Triphan, Tilman, Hesse, Ricarda, Huetteroth, Wolf |
| Publisher | Frontiers Research Foundation |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1662-5153, 640146 |
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