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Stretching and Deforming DNA Molecules in Micro Flows

DNA molecules stretching and deformation in hydrodynamic flow field has been investigated for many years with various experimental and theoretical methods. This research was performed experimentally in hybridized microchannels made of poly(dimethylsiloxane)(PDMS) and glass. We will directly measure the hydrodynamic stretching DNA molecules in elongation flow by CLSM (Confocal Laser Scanning Microscope). The deformation of YOYO-labeled lambda phage DNA molecules in flows was visualized with CLSM. The relation between DNA contour length and relaxation time in different models of stretching flows was also be presented. Furthermore, the distribution of stretching DNA molecules in microchannels was derived to analyze and compared with the DNA migration velocity and stress relaxation modulus.

Identiferoai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0721106-170554
Date21 July 2006
CreatorsLiu, Chang-hao
ContributorsChin-Chia Su, Shou-Shing Hsieh, Shou-Shing Hsieh, Ching-Jenq Ho
PublisherNSYSU
Source SetsNSYSU Electronic Thesis and Dissertation Archive
LanguageCholon
Detected LanguageEnglish
Typetext
Formatapplication/pdf
Sourcehttp://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0721106-170554
Rightsunrestricted, Copyright information available at source archive

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