In this study, an electrokinetics-induced elongation flow was created inside a gradual converging-diverging microchannel with different temperature (25, 35, 45, 55¢XC). The conformation of DNA molecules, local strain rate, and the relaxation time play important roles in determining the extent of DNA stretching. By using £gPIV/£gLIF measurements, the velocity/temperature distributions in microchannels can be secured. The local strain rate was estimated by £gPIV measurements. We observe the hydrodynamic stretching DNA molecules in elongation flow by confocal laser scanning microscope (CLSM). Through CLSM images analysis, relaxation time of DNA molecules was estimated. Finally, dynamic properties and stretching ratio of DNA molecules stretched by EOF driven at various electric field and temperature ware measured. The thermal effect and the electric field on the conformation were also studied and discussed.
| Identifer | oai:union.ndltd.org:NSYSU/oai:NSYSU:etd-0723109-084810 |
| Date | 23 July 2009 |
| Creators | Tsai, Cheng-feng |
| Contributors | Chin-Chia Su, Shou-Shing Hsieh, Ching-Jeng Ho |
| Publisher | NSYSU |
| Source Sets | NSYSU Electronic Thesis and Dissertation Archive |
| Language | Cholon |
| Detected Language | English |
| Type | text |
| Format | application/pdf |
| Source | http://etd.lib.nsysu.edu.tw/ETD-db/ETD-search/view_etd?URN=etd-0723109-084810 |
| Rights | unrestricted, Copyright information available at source archive |
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