Abbrevations
t – fluorescence lifetime
j – rotational correlational time
([Ru(bpy)2(phen-ITC)]2+) – Bis-(bipyridine)-5-(isotiocyanatophenantrolin)-Ru(PF6-)2
Ab – antibody
AF – Alexa Fluor 660
AF-hGH – with Alexa Fluor 660 labelled human growth hormone
FITC – fluorescein isothiocyanate
FITC-Ab – FITC labelled antibody
FP – fluorescence polarization
FRET – fluorescence resonance energy transfer
hGH – human growth hormone
PMI – N-(3-pyrene)maleimide
PMI-hGH – human growth hormone labelled N-(3-pyrene)maleimide
PVPPB – poly(N-vinyl-2-pyrolidone) with phenylboronic acid copolymer
r – anisotropy
RITC – rodamine B isothiocyanate
RITC-hGH – with RITC labeled human growth hormone
SDS-PAGE – sodium dodecylsulfate-polyacrylamide gel electrophoresis
INTRODUCTION
Relevance of the study. Human growth hormone (hGH) is a heterogeneous protein with several molecular forms (isoforms). Human growth hormone measurements are complicated because of the heterogeneous nature of hGH. Specific assays for each isoform are currently unavailable. Because the different antibodies used in immunoassays bind to a different spectrum of hGH isoforms, hGH concentrations measured by immunoassay are likely to depend on the particular antibody used. Moreover, because the distribution of the different hGH isoforms varies among individuals, the results from different immunoassays cannot be interrelated easily by using a single conversion factor.
Fluorescence is by far the most important optical... [to full text]
| Identifer | oai:union.ndltd.org:LABT_ETD/oai:elaba.lt:LT-eLABa-0001:E.02~2006~D_20060517_112604-88755 |
| Date | 17 May 2006 |
| Creators | Jarienė, Giedrė |
| Contributors | Gražulevičius, Juozas Vidas, Maruška, Audrius, Venskutonis, Petras Rimantas, Sadauskienė, Ilona, Marčiulionytė, Dalia, Ivanov, Leonid, Rodovičius, Hiliaras, Kaunas University of Medicine |
| Publisher | Lithuanian Academic Libraries Network (LABT), Kaunas University of Medicine |
| Source Sets | Lithuanian ETD submission system |
| Language | Lithuanian |
| Detected Language | English |
| Type | Doctoral thesis |
| Format | application/pdf |
| Source | http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20060517_112604-88755 |
| Rights | Unrestricted |
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