New and rapid techniques for the characterization and identification of
bacteria would have an important role in clinical microbiology and in food
analysis because of an increasing prevalence of infectious diseases and
In this work we carried out two approaches. In the first study the
characterization and differentiation of mesophilic and thermophilic
bacteria were investigated by using Fourier Transform Infrared (FTIR)
Spectroscopic technique. In the second study, we investigated the
characterization and identification of 3 Bacillus and Micrococcus species
Our results from first approach show that there was a dramatic
difference between mesophilic and thermophilic bacteria. The protein
concentration was high, lipid concentration, the level of triglycerides and the unsaturated acyl chains decreased in thermophilic bacteria. We
found that in thermophilic bacteria PO-
2 groups become hydrogen
bounded. In addition, our results suggest that the cellular DNA content
was low in thermophilic bacteria.
Moreover there were characteristic peaks for both mesophilic and
thermophilic bacteria and these peaks can be used for the differentiation
of these two bacteria group. There were also some specific peaks that
can be used for the differentiation of Escherichia coli and Lactobacillus
plantarum at species level.
In the second approach, our results show that there were significant
spectral differences between Bacillus and Micrococcus species such as
the proportion of unsaturated acyl chains in triglycerides were higher in
Micrococcus species. Moreover we observed different bands that may
be explained by an acetate oxidation via the tricarboxylic acid cycle and
an exopolymer formation in Micrococcus species. In addition to that
another band similar to glycogen, may be explained by a glycogen-like
storage material in Micrococcus species. Also there are characteristic
peaks that can be used for identification of Micrococcus spp.
| Identifer | oai:union.ndltd.org:METU/oai:etd.lib.metu.edu.tr:http://etd.lib.metu.edu.tr/upload/12606673/index.pdf |
| Date | 01 September 2005 |
| Creators | Garip, Sebnem |
| Contributors | Severcan, Feride |
| Publisher | METU |
| Source Sets | Middle East Technical Univ. |
| Language | English |
| Detected Language | English |
| Type | M.S. Thesis |
| Format | text/pdf |
| Rights | To liberate the content for public access |
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