In the early stage of atherosclerosis, monocytes in the circulation become adherent and migrate into the intima of the blood vessel wall in response to stimuli, such as injury and inflammation, and inflammatory factors and metalloproteinases promote this process (Reaven and Witztum 1996). THP-1 monocytic cells have been used as a model to investigate the process of atherosclerosis as they grow in suspension, but become adherent, form processes and produce metalloproteinases and cytokines after phorbol 12-myristate 13-acetate (PMA) treatment. PMA treatment of THP-1 cells results in elevation of steady state levels of MMP9 at 9h, but ADAMTS4 was significantly induced at later time points, which suggested that other factors may be involved in it. The aim of this thesis was to investigate the factors involved in ADAMTS4 and MMP9 regulation.
| Identifer | oai:union.ndltd.org:bl.uk/oai:ethos.bl.uk:502234 |
| Date | January 2009 |
| Creators | Shi, Wei |
| Publisher | University of East Anglia |
| Source Sets | Ethos UK |
| Detected Language | English |
| Type | Electronic Thesis or Dissertation |
Page generated in 0.002 seconds