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Detection and molecular identification of Mucorales isolated from spoilt agricultural commodities collected in fresh produce markets in Gauteng province, South Africa

Fruit and vegetables are often spoilt during storage, handling and transportation due to microorganisms. The common spoilage causes are fungi within the order Mucorales, the largest order of the class Zygomycetes. Such spoilage can result in reduced food supplies, poor quality and severe losses to producers and traders. The study was to investigate the type of Mucorales prevalent in various commodities and in a particular market than others.
Fifty infected papaya, peaches and strawberries were collected at five occasions from large, medium and small markets. Isolation was done aseptically in a biosafety cabinet. Mucorales were identified morphologically, through culture based tests and molecular techniques.
Mucorales isolated are Rhizopus stolonifer, Mucor circinelloides and Mucor racemosus. Mucorales were isolated at a higher rate in samples collected from the small market than other two markets. Spoilage in all three markets is assumed to be influenced by lack of modified temperatures in the storage room. / Life and Consumer Sciences / M. Sc. (Life Sciences)

Identiferoai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:unisa/oai:uir.unisa.ac.za:10500/19632
Date12 1900
CreatorsKwinda, Grace Thiambi
ContributorsRong, Isabella I. H., Jacobs, A., Lebelo, S. L.
Source SetsSouth African National ETD Portal
LanguageEnglish
Detected LanguageEnglish
TypeDissertation
Format1 online resource (xvi, 106 leaves) : illustrations (some color), application/pdf

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