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Previous issue date: 2007-06-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / ?Dengue, amongst the virus illnesses one can get by vectorial transmission, is the one that causes more impact in the morbidity and mortality of world s population. The
resistance to the insecticides has caused difficulties to control of vector insect (Aedes aegypti) and has stimulated a search for vegetables with larvicidal activity. The biodiversity of Caatinga is barely known and it is potential of use even less. Some plants of this biome are commercialized in free fairs northeast of Brazil, because of its phytotherapics properties. The vegetables in this study had been selected by means of a questionnaire applied between grass salesmen and natives of the Serido region from Rio Grande do Norte state; culicids eggs had been acquired with traps and placed in container with water for the larva birth. Thirty larvae had been used in each group (a group control and five experimental groups), with four repetitions four times. The vegetables had been submitted to the processes of decoction, infusion and maceration in the standard concentration of 100g of the vegetable of study in 1l of H2O and analyzed after ?, 1, 2, 4, 8, 12, 24 and 48 hours for verification of the average lethal dose (LD50) from the groups with thirty larva. The LD50 was analyzed in different concentrations (50g/l, 100g/l, 150g/l, 200g/l e 300g/l) of Aspidosperma pyrifolium Mart. 48 extracts of rind, leaf and stem of the seven vegetal species: Aspidosperma pyrifolium Mart., Mimosa verrucosa Benth, Mimosa hostilis (Mart.) ?Benth., Myracrodruon urundeuva Allem?o, Ximenia americana L, Bumelia sartorum Mart Zizyphus joazeiro Mart, had been analyzed. The extracts proceeding from the three methods were submitted to the freezedrying, to evaluate and to quantify substances extracted in each process. The results had shown that Aspidosperma pyrifolium Mart. and Myracrodruon urundeuva Allem?o are the species that are more distinguished as larvicidal after 24 hours of experiment, in all used processes of extraction in the assays. The Zizyphus joazeiro Mart species has not shown larvicidal activity in none of the assays. In relation to the extraction method, the decoction was the most efficient method in the mortality tax of the A. aegypti larvae / ?A Dengue, dentre as doen?as virais de transmiss?o vetorial, ? a que mais causa impacto na morbidade e mortalidade da popula??o mundial. O surgimento de resist?ncia
aos inseticidas tem causado dificuldades no controle do inseto vetor (Aedes aegypti) e estimulado ? busca de vegetais com a??o larvicida. A biodiversidade da caatinga ?
pobremente conhecida e o seu potencial de uso menos ainda. Algumas plantas desse bioma s?o comercializadas em feiras livres do Nordeste do Brasil, com base em suas propriedades fitoter?picas. Os vegetais usados neste estudo foram selecionados por meio de?um?question?rio?aplicado entre?vendedores?de?ervas?e?populares?da?regi?o?do?Serid? do Rio Grande do Norte; ovos de culic?deos foram adquiridos com armadilhas de postura, e colocados em recipiente com ?gua para eclos?o das larvas. Trinta larvas foram usadas em
cada grupo (um grupo controle e cinco grupos experimentais), com quatro repeti??es. Os vegetais foram submetidos aos processos de decoc??o, infus?o e macera??o na
concentra??o padr?o de 200g do vegetal de estudo em 1l de H2O e analisados ap?s ? , 1, 2, 4, 8, 12, 24 e 48 horas para verifica??o da dose letal m?dia (LD50) dos grupos com trinta larvas. A LD50 foi analisada em diferentes concentra??es (50g/l, 100g/l, 150g/l, 200g/l e 300g/l) de Aspidosperma pyrifolium Mart. Foram analisados 48 extratos de casca, folha e caule pertencentes a sete esp?cies vegetais Aspidosperma pyrifolium Mart. (Pereiro), Mimosa verrucosa Benth (Juremabranca), Mimosa hostilis (Mart.) Benth. (Juremapreta), Myracrodruon urundeuva Allem?o (Aroeira), Ximenia americana L (Ameixa), Bumelia sartorum Mart?(Quixabeira), Zizyphus joazeiro Mart?(Joazeiro).?Os extratos?provenientes?dos
tr?s m?todos foram submetidos ? liofiliza??o, para avaliar e quantificar as subst?ncias ?extra?das em cada processo. Os resultados mostraram que Aspidosperma pyrifolium Mart.
e Myracrodruon urundeuva Allem?o s?o as esp?cies que mais se destacam como larvicidas ap?s 24 horas de experimento, em todos os processos de extra??o usados nos
bioensaios. A esp?cie Zizyphus joazeiro Mart n?o demonstrou atividade larvicida em nenhum dos ensaios. Em rela??o ao m?todo de extra??o, a decoc??o foi o m?todo mais
eficaz?na?taxa?de?mortalidade?das?larvas?de A.?aegypti
Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/13040 |
Date | 28 June 2007 |
Creators | Medeiros, Viviane Ferreira de |
Contributors | CPF:20007256434, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4786307T7&dataRevisao=null, Souza, F?bio Santos de, CPF:92771300400, http://lattes.cnpq.br/4903883301058477, Ximenes, Maria de F?tima Freire de Melo |
Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Ci?ncias Biol?gicas, UFRN, BR, Biodiversidade; Biologia Estrutural e Funcional. |
Source Sets | IBICT Brazilian ETDs |
Language | Portuguese |
Detected Language | English |
Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
Format | application/pdf |
Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
Rights | info:eu-repo/semantics/openAccess |
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