The negative epoxy-based photoresist of SU-8 has a variety of applications within microelectromechanical systems (MEMS) and lab-on-a-chip systems. Herein, SU-8 was functionalized with antigenic peptides to HIV-1 gp41 or HIV-2 gp36 and the detection of antibody against HIV-1/2 was carried out by an electrochemical immunoassay combining an alkaline phosphatase conjugated secondary antibody and p-aminophenyl phosphate. The by- product of the reaction (p-aminophenol) was quantitated electrochemically using differential pulse voltammetry, and the current derived from the oxidation of the hydrolysis product increased linearly over a wide primary antibody concentration range (0.001 – 1 μg/mL), with a detection limit of 1 ng mL-1 (6.7 pM) for both HIV-1 and HIV-2. This level of sensitivity is clinically relevant, and feasibility of this approach for clinical sample testing was also evaluated with HIV clinical patient samples.
| Identifer | oai:union.ndltd.org:TORONTO/oai:tspace.library.utoronto.ca:1807/42687 |
| Date | 21 November 2013 |
| Creators | Bhimji, Alyajahan |
| Contributors | Kelley, Shana O. |
| Source Sets | University of Toronto |
| Language | en_ca |
| Detected Language | English |
| Type | Thesis |
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