Seed development, germination and dormancy, considered as the most important phenomena in seeds, are regulated by several plant hormones; gibberellin (GA) and abscisic acid (ABA) being the major players acting antagonistically. The regulation of these seed related processes by GA and ABA is dependent partly on the endogenous levels of the two hormones, which in turn are determined by the balance between their biosynthesis and catabolism. This thesis investigated the spatial and temporal expression patterns of several members of the GA and ABA biosynthetic and catabolic gene families during grain development using a non-dormant cultivar and during imbibition using grains collected from dormant and non-dormant cultivars of barley. In addition, the thesis examined the effect of exogenous ABA treatment, and after-ripening of seeds collected from dormant cultivars on the expression patterns GA and ABA metabolism genes during grain development and imbibition, respectively. The results suggest that specific members of the gene families related to the metabolic pathways of the two hormones exhibit distinct spatial and temporal roles in the regulation of barley grain development, dormancy and germination. / October 2015
| Identifer | oai:union.ndltd.org:MANITOBA/oai:mspace.lib.umanitoba.ca:1993/30790 |
| Date | 14 September 2015 |
| Creators | Park, Seokhoon |
| Contributors | Ayele, Belay T. (Plant Science), Stasolla, Claudio (Plant Science) Hausner, Georg (Microbiology) |
| Source Sets | University of Manitoba Canada |
| Detected Language | English |
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