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Previous issue date: 2008-08-30 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Pleomorphic adenoma and adenoid cystic carcinoma represent a benign and malignant salivary gland neoplasm, respectively, that shares the same histological origin, however with distinct biological behavior. The aim of the present study was identify the -160 C/A
polymorphism in the gene CDH1, mutational analysis of CTNNB1 gene and evaluation the expression of the E-cadherin and ?-catenin in pleomorphic adenomas and adenoid cystic carcinomas. Furthermore, it was proposed correlate the immunochemistry staining patterns with the polymorphism and mutations. Twenty-four pleomorphic adenomas and 24 adenoid
cystic carcinomas were retrieved. The polymorphism analysis was performed by restriction fragment length polymorphism (RFLP), using the restriction enzymes HphI or AflIII and the mutational screening was performed by PCR-single strand conformational polymorphism
(PCR-SSCP). The immunohistochemical analysis was taken by the counting of cells, recorded as the Hscore index, and considering the presence or absence, intensity, distribution and localization of proteins expression. Comparing the two neoplasms, the results demonstrated statistically significant difference for the E-cadherin and ?-catenin expression, with pleomorphic adenoma presenting weaker immunostaining. Was observed statistical correlation between E-cadherin and ?-catenin expression. CDH1 heterozigotic polymorphism was seen in two cases and 13 cases displayed abnormal mobility electrophoretic shifts, suggesting CTNNB1 gene mutation. The immunohistochemical expression was not statistically correlated with the polymorphism or suggested mutations. In conclusion this study supports that the E-cadherin/?-catenin complex immunohistochemical expression might be related with the myoepithelial component amount and differentiation neither the tumor biological behavior. The cases that showed E-cadherin gene polymorphism presented
reduced protein expression and, moreover, CTNNB1 suggested mutations seem not influence in the ?-catenin protein expression / O adenoma pleom?rfico e o carcinoma aden?ide c?stico representam neoplasias de gl?ndula salivar benigna e maligna, respectivamente, que compartilham a mesma origem histol?gica, por?m com comportamentos biol?gicos distintos. O prop?sito deste estudo consistiu na identifica??o do polimorfismo -160 C/A da regi?o promotora do gene CDH1 (E-caderina), na triagem de muta??es no gene CTNNB1 (?-catenina), e ainda na an?lise da express?o imuno-histoqu?mica das prote?nas E-caderina e ?-catenina em adenomas pleom?rficos e carcinomas aden?ides c?sticos. Al?m disso, objetivou-se correlacionar os achados imuno-histoqu?micos com
as poss?veis muta??es e polimorfismo. Foram selecionados 24 casos de adenoma pleom?rfico e 24
casos de carcinoma aden?ide c?stico. Para a identifica??o do polimorfismo no gene da E-caderina empregou-se a t?cnica RFLP (restriction fragment length polymorphism) utilizando-se enzimas de restri??o HphI e AflIII. A triagem de muta??es no exon 3 do gene da ?-catenina foi realizada por meio de SSCP (single strand conformational polymorphism). Para a an?lise imuno-histoqu?mica,
procedeu-se contagem de c?lulas, por meio do ?ndice HScore e verificou-se presen?a ou aus?ncia, intensidade, padr?o de distribui??o e localiza??o celular e tecidual das prote?nas. Os resultados demonstraram diferen?a estatisticamente significativa quando a marca??o imuno-histoqu?mica, tanto da E-caderina quanto ?-catenina, foi comparada entre as duas neoplasias estudadas, apresentando o adenoma pleom?rfico express?o reduzida. Observou-se correla??o estatisticamente significativa entre a imuno-marca??o da E-caderina e ?-catenina. Dois casos (1 adenoma pleom?rfico e 1 carcinoma aden?ide c?stico) apresentaram polimorfismo heterozig?tico no gene CDH1 e 13 casos (6 adenomas pleom?rficos e 7 carcinomas aden?ides c?sticos) exibiram varia??o no padr?o de corrida eletrofor?tica, sugerindo muta??o do gene CTNNB1. N?o houve
correla??o estatisticamente significativa entre a marca??o imuno-histoqu?mica e presen?a de polimorfismo ou poss?veis muta??es. Conclui-se que a express?o imuno-histoqu?mica do complexo E-caderina/?-catenina pode estar relacionada com a quantidade e diferencia??o do componente mioepitelial e n?o ao comportamento biol?gico dos tumores. Os casos que exibiram polimorfismo no gene da E-caderina apresentaram redu??o na express?o prot?ica e, por fim, as poss?veis muta??es no gene CTNNB1 parecem n?o influenciar na express?o da prote?na ?-catenina
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/17140 |
| Date | 30 August 2008 |
| Creators | Cavalcante, Roberta Barroso |
| Contributors | http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787927Z7&dataRevisao=null, Sobral, Ana Paula Veras, CPF:74460536400, http://lattes.cnpq.br/7338874761603515, Rabenhorst, Silvia Helena Baren, CPF:98317130878, http://lattes.cnpq.br/3868264006150535, Freitas, Roseana de Almeida, CPF:28444361453, http://lattes.cnpq.br/9512014003639405, Souza, L?lia Batista de |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Patologia Oral, UFRN, BR, Odontologia |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/doctoralThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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