Subcutaneous (sc) and visceral (vis) adipose tissue (AT) contribute to the variability
in pathophysiological consequences of obesity and adverse fat distribution. To gain
insights into the molecular mechanisms distinguishing vis and sc fat, we compared the
transcriptome during differentiation of immortalized adipocytes from murine epididymal
(epi) and inguinal (ing) AT. RNA was extracted on different days of adipogenesis (−2, 0, 2,
4, 6, 8) and analyzed using ClariomTM D mouse assays (Affymetrix) covering >214,900
transcripts in >66,100 genes. Transcript Time Course Analysis revealed 137 differentially
expressed genes. The top genes with most divergent expression dynamics included
developmental genes like Alx1, Lhx8, Irx1/2, Hoxc10, Hoxa5/10, and Tbx5/15. According
to pathway analysis the majority of the genes were enriched in pathways related to AT
development. Finally, in paired samples of human vis and sc AT (N = 63), several of
these genes exhibited depot-specific variability in expression which correlated closely
with body mass index and/or waist-to-hip ratio. In conclusion, intrinsically programmed
differences in gene expression patterns during adipogenesis suggest that fat depot
specific regulation of adipogenesis contributes to individual risk of obesity.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84553 |
| Date | 04 April 2023 |
| Creators | Breitfeld, Jana, Kehr, Stephanie, Müller, Luise, Stadler, Peter F., Böttcher, Yvonne, Blüher, Matthias, Stumvoll, Michael, Kovacs, Peter |
| Publisher | Frontiers Research Foundation |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 1664-2392, 138 |
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