Studies have shown that overexpression of C1q TNF-related protein-3 (CTRP3) in mice fed a high alcohol diet can protect the animal from developing alcoholic fatty liver disease (ALD). Preliminary data from a recent study, however, indicated that overexpression of CTRP3 coupled with a high fat diet may cause kidney damage, including the development of (apparent) hyperlipidemic renal tubular vacuolization. If these findings are correct, using CTRP3 as a treatment for ALD would require careful monitoring of the patient diet to insure they limited the amount of fat in their diet. This recent study, however, utilized paraffin embedded kidney tissue. The processing required for paraffin embedding removes lipids/fat from the tissue. Thus, it is possible that what were assumed to be fat vacuoles in the kidney tubules may have been vacuoles unrelated to fat deposition, which represent a different aspect of tissue damage. Adipophilin and/or perilipin are proteins found in the membranes of lipocytes and membrane bound vesicles of fat. Immunohistochemical staining for adipophilin and/or perilipin 1 was used to determine if the damage seen in kidneys from the previous study was related to fat deposition or some other damaging factor. This information can inform further studies to determine how to proceed to safely administer CTRP3 to patients with ALD without damaging their kidneys.
| Identifer | oai:union.ndltd.org:ETSU/oai:dc.etsu.edu:asrf-2149 |
| Date | 25 April 2023 |
| Creators | Wren, Noah, Nisar, Ummah Salma, Youngberg, George, Forsman, Allan |
| Publisher | Digital Commons @ East Tennessee State University |
| Source Sets | East Tennessee State University |
| Detected Language | English |
| Type | text |
| Source | Appalachian Student Research Forum |
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