Populations at risk for tuberculosis (TB) may have a low n-3 polyunsaturated fatty acid
(PUFA) status. Our research previously showed that post-infection supplementation of
n-3 long-chain PUFA (LCPUFA) in TB without TB medication was beneficial in n-3
PUFA sufficient but not in low-status C3HeB/FeJ mice. In this study, we investigated
the effect of n-3 LCPUFA adjunct to TB medication in TB mice with a low compared
to a sufficient n-3 PUFA status. Mice were conditioned on an n-3 PUFA-deficient (n-
3FAD) or n-3 PUFA-sufficient (n-3FAS) diet for 6 weeks before TB infection. Postinfection
at 2 weeks, both groups were switched to an n-3 LCPUFA [eicosapentaenoic
acid (EPA)/docosahexaenoic acid (DHA)] supplemented diet and euthanized at 4- and
14- days post-treatment. Iron and anemia status, bacterial loads, lung pathology, lung
cytokines/chemokines, and lung lipid mediators were measured. Following 14 days of
treatment, hemoglobin (Hb) was higher in the n-3FAD than the untreated n-3FAS group
(p = 0.022), whereas the n-3FAS (drug) treated control and n-3FAS groups were not. Proinflammatory
lung cytokines; interleukin-6 (IL-6) (p = 0.011), IL-1a (p = 0.039), MCP1
(p = 0.003), MIP1- a (p = 0.043), and RANTES (p = 0.034); were lower, and the antiinflammatory
cytokine IL-4 (p=0.002) and growth factor GMCSF (p=0.007) were higher
in the n-3FAD compared with the n-3FAS mice after 14 days. These results suggest that
n-3 LCPUFA therapy in TB-infected mice, in combination with TB medication, may
improve anemia of infection more in low n-3 fatty acid status than sufficient status mice.
Furthermore, the low n-3 fatty acid status TB mice supplemented with n-3 LCPUFA
showed comparatively lower cytokine-mediated inflammation despite presenting with
lower pro-resolving lipid mediators.
| Identifer | oai:union.ndltd.org:DRESDEN/oai:qucosa:de:qucosa:84352 |
| Date | 28 March 2023 |
| Creators | Hayford, Frank E. A., Dolman, Robin C., Ozturk, Mumin, Nienaber, Arista, Ricci, Cristian, Loots, Du Toit, Brombacher, Frank, Blaauw, Renée, Smuts, Cornelius M., Parihar, Suraj O., Malan, Linda |
| Publisher | Frontiers Research Foundation |
| Source Sets | Hochschulschriftenserver (HSSS) der SLUB Dresden |
| Language | English |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, doc-type:article, info:eu-repo/semantics/article, doc-type:Text |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2296-861X, 695452 |
Page generated in 0.0016 seconds