Objetivo: Evaluar in vitro el efecto antibacteriano de cuatro extractos metanólicos de Macrocystis pyrifera, Lessonia trabeculata, Lessonia nigrescens y Chondracanthus chamissoi sobre Streptococcus mutans (ATCC®35668™). Metodología: El presente estudio fue de tipo experimental in vitro, en el cual se prepararon cuatro extractos metanólicos de Macrocystis pyrifera, Lessonia trabeculata, Lessonia nigrescens y Chondracanthus chamissoi. Para cada extracto se realizaron 6 pruebas independientes y como control positivo se utilizó la solución de Clorhexidina al 0.12%. Para evaluar el efecto antibacteriano de los extractos se utilizó el método de difusión en pozo de Kirby-Bauer. La concentración mínima inhibitoria (CMI) y la concentración mínima bactericida (CMB) fueron evaluadas mediante el método de microdilución y el efecto citotóxico fue determinado por el método de MTT. Resultados: Se observó mayor efecto antibacteriano de los extractos metanólicos de Lessonia trabeculata y Macrocystis pyrifera sobre cepas de Streptococcus mutans, mostrando halos de inhibición de 18.1 mm + 0.75 mm y 12.25 mm + 0.69 mm respectivamente. Mientras que los extractos metanólicos de Lessonia nigrescens y Chondracanthus chamissoi mostraron menor efecto antibacteriano sobre cepas de Streptococcus mutans, observándose halos de 10.08 mm + 0.46 mm y 9.32 mm + 0.49 mm respectivamente. En todos los casos, los extractos metanólicos muestran una baja citoxicidad sobre células MDCK, observando una viabilidad celular de 66% para el caso de Lessonia trabeculata, 85% para el caso de Chondracanthus chamissoi, 65% para el caso de Lessonia nigrescens y 79% para el caso de Macrocystis pyrifera. Conclusiones: Se determinó que los cuatro extractos metanólicos de Macrocystis pyrifera, Lessonia trabeculata, Lessonia nigrescens y Chondracantus chamissoi presentan efecto antibacteriano sobre cepas de Streptococcus mutans, mostrando diferencias significativas entre ellos. Además, los cuatro extractos metanólicos presentan bajo efecto citotóxico sobre MDCK a altas concentraciones. / Objective: The aim of this study was to evaluate the antibacterial of the methanolic extracts of Macrocystis pyrifera, Lessonia trabeculata, Lessonia nigrescens and Chondracanthus chamissoi againts Streptococcus mutans (ATCC ®35668™). Material and methods: The present study was of experimental type in vitro, in which four methanolic extracts of Macrocystis pyrifera, Lessonia trabeculata, Lessonia nigrescens and Chondracanthus chamissoi were prepared. For each extract 6 independent tests were performed and as a positive control, the 0.12% Chlorhexidine solution was used. He went with the method of diffusion in the well of Kirby-Bauer, then proceeded to the reading of the halos with an external cover Vernier. Likewise, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (CMB) of the extracts were found and the cytotoxic effect of each methanolic extract was determined with the MTT method. Results: A higher antibacterial effect was found in the methanolic extracts of Lessonia trabeculata and Macrocystis pyrifera with halos of 18.1 mm + 0.75 mm and 12.25 mm + 0.69 mm respectively on Streptococcus mutans respectively; however, it has the minor antibacterial effect of the methanolic extracts of Lessonia nigrescens and Chondracanthus chamissoi with halos of 10.08 mm + 0.46 mm and 9.32 mm + 0.49 mm. In cell viability, Lessonia trabeculata showed an effect at 66% of concentration similar to or less than 10000 mg / ml of the extract, while the extract of Chondracanthus chamissoi showed an effect superior to 84%, the extract of Lessonia nigrescens to 65% and the extract derived from Macrocystis pyrifera at 79%. Conclusions: It was determined that the four algae have an antibacterial effect against Streptococcus mutans, however there is a statistically significant difference between the extracts of Macrocystis pyrifera and Lessonia trabeculata with the extracts of Lessonia nigrescens and Chondracantus chamissoi. Likewise, the four algae have low cytotoxic effect at high concentrations. / Tesis
| Identifer | oai:union.ndltd.org:PERUUPC/oai:repositorioacademico.upc.edu.pe:10757/624450 |
| Date | 01 September 2018 |
| Creators | Cavero Tasayco, Maryori Alexandra, Angeles Bonelli, Gianella Solange |
| Contributors | del Valle Mendoza, Juana |
| Publisher | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Source Sets | Universidad Peruana de Ciencias Aplicadas (UPC) |
| Language | Spanish |
| Detected Language | English |
| Type | info:eu-repo/semantics/bachelorThesis |
| Format | application/pdf, application/epub, application/msword |
| Source | Universidad Peruana de Ciencias Aplicadas (UPC), Repositorio Académico - UPC |
| Rights | info:eu-repo/semantics/embargoedAccess, Attribution-NonCommercial-ShareAlike 3.0 United States, http://creativecommons.org/licenses/by-nc-sa/3.0/us/ |
Page generated in 0.003 seconds