Thesis (M.Sc. (Zoology)) --University of Limpopo, 2012 / A microsatellite-based study was performed on five populations of Gemsbok (Oryx gazella). This study was aimed at estimating genetic diversity in introduced South African gemsbok populations (an opportunity that arose when additional animals from the same source were imported into South Africa), and determine genetic structure. Population sizes at the time of sampling were: Namibia (n = 6500), Cohen (n = 70), Tempelhof (n = 55), STS Kalahari Game Ranch (n = 1000) and Elias (n = 35). The purpose of the study was to determine the genetic structure of the aforementioned O. Gazelle populations, and to assess the impact of the founder effect on isolated populations. The following primers (BMS1237, MAF46, OARFC304, OARHH64, ETH225, RBP3, MAF50, HDZ8) developed for commercial purposes in the bovine group were used. Genetic diversity were calculated as Expected Heterozygosity (He), proportion of polymorphic loc (P) and number of alleles per locus (A). Conformation to expected Hardy-Weinberg equilibrium of genotypes was also determined, using a Chi-square test. Tests for the signature of bottlenecks in the populations studied were also performed. Genetic drift/differentiation was tested by using FST and RST coefficients. Assignment tests were performed to identify the true number of genetic populations (clusters). Genetic distance was used as an additional measure of differentiation. The results indicated that all loci showed allelic polymorphism in all the populations except one (at the OARHH64 locus). The South African Cohen population displayed the highest level of genetic diversity, with He = 0.595 ± 0.247. This population also did not show evidence of a bottleneck. Genetic distance values indicated the greatest similarity between the Cohen and Namibian populations, in line with the Namibian origin of the Cohen group. Greatest distance was observed between the STS and Tempelhof populations. conclusion, results from this study reflects the origins of populations and suggest that inbreeding in small isolated populations may be less than previously estimated.
| Identifer | oai:union.ndltd.org:netd.ac.za/oai:union.ndltd.org:ul/oai:ulspace.ul.ac.za:10386/746 |
| Date | January 2012 |
| Creators | Osmers, Karl Benjamin |
| Contributors | Grobler, J.P., Engelbrecht, G.D. |
| Publisher | University of Limpopo (Turfloop Campus) |
| Source Sets | South African National ETD Portal |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | 107 leaves, map |
| Relation |
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