Weathering of silicate minerals has long been a known source of natural CO2 sequestration, that could be increased in the presence of microorganisms. Bio-enhanced weathering of silicate minerals could increase the sequestration of CO2 from the atmosphere. The aim of this project was to evaluate the potential for a new Neutral emission technology (NET), using four different organisms, Aspergillus Niger, Knufia Petricola, Bacillus Subtilis and Cupriavidus Metallidurans and their potential to increase olivine weathering (dunite). Straw, manure and digestate was used as carbon sources. In total 9 biotic - and 9 abioitc reactors were made, containing a mixture of dunite and one of the three carbon sources. In total 250 mL of water was added to each reactor per week, for 6 weeks, and collected at the end of the week for analysis. Geochemical analyses of the leachate were performed, including pH, conductivity, alkalinity, total organic carbon (TOC), total inorganic carbon (TIC), cations, anions and three organic acids: citrate, acetate, and oxalate. Scanning emission microscope (SEM) was used to monitor potential differences pre- and post-treatment. Straw reactors produced the most growth, both on the carbon source and the dunite grains. Likely due to the increased labile organic carbon concentrations. The total inorganic carbon and alkalinity demonstrated that inoculation of the reactors promoted weathering for all carbon sources, most significantly for the straw reactors. This observation was evidenced by etch pits in the SEM images and higher TIC, alkalinity, and magnesium values. Microbially enhanced silicate weathering has demonstrated it could be used for the development of NETs for the sequestration of atmospheric carbon. / BAM!
| Identifer | oai:union.ndltd.org:UPSALLA1/oai:DiVA.org:uu-479950 |
| Date | January 2022 |
| Creators | Westholm, Marcus |
| Publisher | Uppsala universitet, Limnologi |
| Source Sets | DiVA Archive at Upsalla University |
| Language | English |
| Detected Language | English |
| Type | Student thesis, info:eu-repo/semantics/bachelorThesis, text |
| Format | application/pdf |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | UPTEC X ; 22024 |
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