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Previous issue date: 2012-12-21 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This study examines the physical and chemical composition and the pharmacological
effects of brown seaweed FRF 0.8 Lobophora variegata. Fractionation of the crude
extract was done with the concentration of 0.8 volumes of acetone, obtaining the
FRF 0.8. The physicochemical characterization showed that it was a fucana sulfated.
Anti-inflammatory activity was assessed by paw edema model by the high rates of
inhibition of the edema and the best results were in the fourth hour after induction
(100 ? 1.4% at the dose of 75 mg / kg) and by the strong inhibitory activity of the
enzyme myeloperoxidase (91.45% at the dose of 25 mg / kg). The hepataprote??o
was demonstrated by measurements of enzymatic and metabolic parameters
indicative of liver damage, such as bilirubin (reduction in 68.81%, 70.68% and
68.21% for bilirubin total, direct and indirect, respectively at a dose of 75 mg / kg),
ALT, AST and γ-GT (decrease of 76.93%, 44.58% and 50% respectively at a dose of
75 mg / kg) by analysis of histological slides of liver tissue, confirming that
hepatoprotective effect the polymers of carbohydrates, showing a reduction in tissue
damage caused by CCl4 and the inhibition of the enzyme complex of cytochrome P
450 (increasing sleep time in 54.6% and reducing the latency time in 71.43%). The
effectiveness of the FRF 0.8 angiogenesis was examined in chorioallantoic
membrane (CAM) of fertilized eggs, with the density of capillaries evaluated and
scored, showing an effect proangig?nico at all concentrations tested FRF (10 mg-
1000 mg). The FRF showed antioxidant activity on free radicals (by inhibiting
Superoxide Radical in 55.62 ? 2.10%, Lipid Peroxidation in 100.15 ? 0.01%, Hydroxyl
Radical in 41.84 ? 0.001% and 71.47 Peroxide in ? 2.69% at concentration of 0.62
mg / mL). The anticoagulant activity was observed with prolongation of activated
partial thromboplastin time (aPTT) at 50 mg (> 240 s), showing that its action occurs
in the intrinsic pathway of the coagulation cascade. Thus, our results indicate that
these sulfated polysaccharides are an important pharmacological target / Este estudo analisa a composi??o f?sico-qu?mica e os efeitos farmacol?gicos da FRF
0,8 da alga marrom Lobophora variegata. O fracionamento do extrato bruto foi feito
com a concentra??o de 0,8 volumes de acetona, obtendo a FRF 0,8. A
caracteriza??o f?sico-qu?mica mostrou que se tratava de uma fucana sulfatada. Foi
verificada a atividade antinflamat?ria pelo modelo de edema de pata, atrav?s das
altas taxas de inibi??o do edema e os melhores resultados foram na quarta hora
ap?s a indu??o (100 ? 1,4% com a dose de 75 mg/kg) e pela forte atividade inibidora
da enzima mieloperoxidase (91,45% com a dose de 25 mg/kg). A hepataprote??o foi
demonstrada pelas dosagens de par?metros metab?licos e enzim?ticos indicativos
de dano hep?tico, como bilirrubina (redu??o em 68,81%, 70,68% e 68,21% para
bilirrubinas total, direta e indireta, respectivamente na dose de 75 mg/kg), ALT , AST
e γ-GT (diminui??o em 76,93%, 44,58% e 50%, respectivamente na dose de 75
mg/kg), pela an?lise das l?minas histol?gicas do tecido hep?tico, que confirmam
esse efeito hepatoprotetor dos pol?meros de carboidrato, mostrando uma redu??o no
dano tecidual causado por CCl4, e pela inibi??o do complexo enzim?tico do
citocromo P 450 (aumentando o tempo de sono em 54,6% e reduzindo o tempo de
lat?ncia em 71,43%). A efic?cia sobre a angiogenese da FRF 0,8 foi examinada na
membrana corioalant?ica (CAM) de ovos fertilizados, com a densidade dos capilares
avaliadas e pontuadas, mostrando um efeito proangig?nico em todas as
concentra??es de FRF testadas (10 μg-1000 μg). A FRF apresentou a??o
antioxidante sobre radicais livres (inibindo o Radical Super?xido em 55,62?2,10%,
Peroxida??o Lip?dica em 100,15?0,01%, Radical Hidroxila em 41,84?0,001% e
Per?xido em 71,47?2,69%, todos na concentra??o de 0,62 mg/mL). A atividade
anticoagulante foi verificada com o prolongamento do tempo de tromboplastina
parcial ativada (aPTT) a 50 μg (>240 s), mostrando que sua a??o ocorre na via
intr?nseca da cascata de coagula??o. Sendo assim, nossos resultados indicam que
estes polissacar?deos sulfatados constituem um importante alvo farmacol?gico
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/12598 |
| Date | 21 December 2012 |
| Creators | Will, Luiza Sheyla Evenni Porf?rio |
| Contributors | CPF:06341462468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783254U6&dataRevisao=null, Dore, Celina Maria Pinto Guerra, CPF:03383860418, http://lattes.cnpq.br/2279068301048550, Paiva, Patr?cia Maria Guedes, CPF:36565431434, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787593U2&dataRevisao=null, Leite, Edda Lisboa |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
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