Made available in DSpace on 2015-03-03T13:58:18Z (GMT). No. of bitstreams: 1
AlminoAOP_DISSERT.pdf: 2473203 bytes, checksum: bef6a29e74144b9845b6ff4ab8398a69 (MD5)
Previous issue date: 2010-09-13 / Fucans seaweed Lobophora variegata estructures are known for their chemical
and biological properties. In this study, we analyzed, the action of fucans L.
variegata and the fractions purified with acetone in Zymosan-induced arthritis.
After differential fractionation with acetone, six fractions were obtained and
named F0.3, F0.5, F0.8, F1, F1.5 and F2. The results showed that the fraction
F1 showed high yield (51.9%) and was chosen for studies of antioxidant activity
and induced arthritis. Nuclear magnetic resonance (NMR) of 13C showed
signals at 103.3 and 15.78 ppm that are assigned to links ?13 galactose and
of the C6 methyl fucose, respectively. The infrared (IR) showed absorbance at
1238 and 850 cm-1 which are attributed to sulfate. The fraction F1 showed
antioxidant activities in vitro. For analysis of inflammatory parameters chosen
the polysaccharide was administered in different doses (25, 50 and 75 mg / kg
ip, per body weight) and diclofenac sodium (5 mg / kg ip) and L-NAME (25 mg /
kg ip) in groups of animals (n = 6). After 6 h, were analyzed for cellular influx
and levels of nitrite. In experiment five days, were made analysis of swelling
and serum TNF-?. Histopathological analysis were performed for confirmation
of results. The fraction F1 (25, 50 and 75 mg / kg ip) reduced the cellular influx
(52.1 to 96.7%) and nitric oxide levels (27.2 - 39%) compared to control group.
The reduction of edema (63.4 - 100%) and serum TNF-? (p <0.001) were
observed when the polysaccharide F1 administered at a dose (50 mg / kg)
These results suggest that these heterofucanas of Lobophora variegata have
besides the activity antioxidant and potential anti-inflammatory activity in
arthritis induced by zymosan / Fucanas da alga Lobophora variegata s?o conhecidas por suas estruturas
qu?micas e propriedades biol?gicas. Nesse estudo, analisou-se a a??o de
fucanas de L. variegata e suas fra??es purificadas com acetona na artrite
induzida por Zymosan. Ap?s fracionamento diferencial com acetona, 6 fra??es
foram obtidas e nomeados F0.3, F0.5, F0.8, F1, F1.5 e F2. Os resultados
mostraram que a fra??o F1 apresentou alto rendimento (51,9%) e foi escolhida
para estudos da atividade antioxidante e artrite induzida. A resson?ncia
magn?tica nuclear (RMN) de 13C mostrou sinais a 103,3 e 15,78 ppm que s?o
atribu?dos ?s liga??es ?1 3 da galactose e metil do C6 da fucose,
respectivamente. O infravermelho (IV) mostrou absorb?ncia a 1238 e 850 cm-1
que s?o atribu?das ao sulfato. A fra??o F1 apresentou atividades antioxidantes
in vitro. Para an?lise de par?metros inflamat?rios a fra??o polissacar?dica
escolhida foi administrada em diferentes doses (25, 50 e 75 mg/kg i.p, por peso
corporal), assim como diclofenaco de s?dio (5 mg/kg i.p.) e L-NAME (25 mg/kg
i.p.) em grupos de animais (n=6). Depois de 6 h, foram realizadas an?lises de
influxo celular e n?veis de nitrito. Em experimento de cinco dias, foram
efetuadas analises de edema e TNF-? s?rico. An?lises histopatol?gicas foram
realizadas para confirma??o de resultados. A fra??o F1 (25, 50 e 75 mg/kg i.p.)
reduziu o influxo celular (52,1 ? 96,7%) e os n?veis ?xido n?trico (27,2 ? 39%)
em rela??o ao grupo controle. A redu??o do edema (63,4 - 100%) e TNF-?
s?rico (p < 0,001) foram observadas quando administrado o polissacar?deo F1
na dosagem (50 mg/kg) Esses resultados sugerem que essas heterofucanas
de Lobophora variegata possuem al?m da atividade antioxidante, potencial
atividade anti-inflamat?ria na artrite induzida por zymosan
| Identifer | oai:union.ndltd.org:IBICT/oai:repositorio.ufrn.br:123456789/18532 |
| Date | 13 September 2010 |
| Creators | Paiva, Almino Afonso de Oliveira |
| Contributors | CPF:06341462468, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783254U6&dataRevisao=null, Jer?nimo, Selma Maria Bezerra, CPF:15603016434, http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785584A3&dataRevisao=null, Carvalho, Maria Goretti Freire de, CPF:05601592420, http://lattes.cnpq.br/8934375314306198, Leite, Edda Lisboa |
| Publisher | Universidade Federal do Rio Grande do Norte, Programa de P?s-Gradua??o em Bioqu?mica, UFRN, BR, Bioqu?mica; Biologia Molecular |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Repositório Institucional da UFRN, instname:Universidade Federal do Rio Grande do Norte, instacron:UFRN |
| Rights | info:eu-repo/semantics/openAccess |
Page generated in 0.0032 seconds